Team:UNICAMP-Brazil/Notebooks/September 2

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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====Lysozyme PCR==== The results obtained today were extremely awaited. We di...)
(RBS+CFP ligation)
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====RBS+CFP ligation====
====RBS+CFP ligation====
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‘‘’Electrophoresis of digestion made yesterday (digestion of the plasmids containing RBS+CFP parts) ‘’’
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We verified using electrophoresis, the results of the digestion made yesterday (RBS+CFP plasmids). The gel showed we had no success with the digestions.
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The gel showed not success with the digestions.  
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''Wesley''
''Wesley''
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{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 20:27, 7 September 2009

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YeastGuard

Lysozyme PCR

The results obtained today were extremely awaited. We did the PCR using the lysozyme primers. There was a total of 13 reactions: one for each organ, using the 2 cDNAs and the RNA without treatment (because there was still genomic in it!).

GEL PCR

As you can see in the gel, we got specific bands from the oviduct sample! =]

Taís

RBS+CFP ligation

We verified using electrophoresis, the results of the digestion made yesterday (RBS+CFP plasmids). The gel showed we had no success with the digestions.

Wesley