Team:UNICAMP-Brazil/Notebooks/September 30
From 2009.igem.org
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* After confirming the miniprep procedure, we digested the cited biobricks with the correct enzymes in order to make possible the construction of our necessary devices. Therefore, we had to: | * After confirming the miniprep procedure, we digested the cited biobricks with the correct enzymes in order to make possible the construction of our necessary devices. Therefore, we had to: | ||
- | - BBa_E0840: isolate the part in order to ligate it in the | + | - BBa_E0840: isolate the part in order to ligate it in the downstream position of another biobrick (digested with XpeI and PstI) |
- BBa_I718017: open the biobrick in the downstream position of it's part (digested with SpeI and PstI) | - BBa_I718017: open the biobrick in the downstream position of it's part (digested with SpeI and PstI) | ||
- | - BBa_J61000: isolate the part in | + | - BBa_J61000: isolate the part in order to insert it in the upstream position of another biobrick (digested with EcoRI and SpeI) |
- BBa_I718016: open the biobrick in the upstream position of it's part (digested with EcoRI and XbaI) | - BBa_I718016: open the biobrick in the upstream position of it's part (digested with EcoRI and XbaI) |
Revision as of 17:29, 18 October 2009
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