Team:UNICAMP-Brazil/Notebooks/September 4

(Difference between revisions)

Revision as of 00:24, 18 October 2009

Today we prepared electrocompetent E. coli to use in our transformations according to Protocol 4.

Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction. We ressuspended the biobrick of Cre-Recombinase: BBa_J61047 and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.

Víctor

@@ Line 19: / Line 19: @@
 {{:Team:UNICAMP-Brazil/inc_rodape}}
-==== PY Promoter ====
-*<p style=”text-align:justify;”>Today we performed the PCR amplification of the PY promoter from the F plasmid previously extracted from the conjugative strain. We did two different reactions, using two types of forward primers (Ppy-F-1 and Ppy-F-2) for the same reverse primer (Ppy-R). The product amplified with the Ppy-F-1/Ppy-R primers has 133 bp and the one amplified with the Ppy-F-2/Ppy-R has 75 bp. We called these products PY1 and PY2 respectively. The sizes of the products were confirmed by an agarose gel:
-''Fabiana and Leonardo''