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Team:UNICAMP-Brazil/Notebooks/September 4
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====Electrocompetent ''E. coli''==== | ====Electrocompetent ''E. coli''==== | ||
| - | *Today we prepared electrocompetent ''E. coli'' to use in our transformations according to Protocol 4 | + | *<p style=”text-align:justify;”>Today we prepared electrocompetent ''E. coli'' to use in our transformations according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Preparation_of_electrocompetent_E._coli Protocol 4].</p> |
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==''' ColiGuards '''== | ==''' ColiGuards '''== | ||
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==== Cre-Recombinase without ATG ==== | ==== Cre-Recombinase without ATG ==== | ||
| - | *Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction. We ressuspended the biobrick of Cre-Recombinase: BBa_J61047 and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page. | + | *<p style=”text-align:justify;”>Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction. We ressuspended the biobrick of Cre-Recombinase: BBa_J61047 and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.</p> |
''Víctor'' | ''Víctor'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 22:51, 3 October 2009
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