Team:UNICAMP-Brazil/Notebooks/September 4

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MicroGuards

Electrocompetent E. coli

  • Today we prepared electrocompetent E. coli to use in our transformations according to Protocol 4.

ColiGuards

Cre-Recombinase without ATG

  • Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction. We ressuspended the biobrick of Cre-Recombinase: BBa_J61047 and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.

Víctor