Team:UNICAMP-Brazil/Yeastguard/Results

From 2009.igem.org

(Difference between revisions)
(→Yeast experiments: Lysozyme)
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===We can build and confirm the following Biobricks:===
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===We confirm the following Biobricks:===
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''Parts:''
''Parts:''
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* JEN1 Promoter from Kluyveromyces lactis
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* BBa_K284002 - JEN1 Promoter from Kluyveromyces lactis -
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* Partial DLD Promoter from Kluyveromyces lactis
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* BBa_K284003 - Partial DLD Promoter from Kluyveromyces lactis
''Devices to JEN1 and DLD promoters characterization:''
''Devices to JEN1 and DLD promoters characterization:''
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* EYFP regulated by lactate responsive promoter (JEN1)
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* BBa_K284020 - EYFP regulated by lactate responsive promoter (JEN1)
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* EYFP regulated by lactate responsive promoter (DLD)
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* BBa_K284021 - EYFP regulated by lactate responsive promoter (DLD)
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* EYFP regulated by constitutive promoter Adh1
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* BBa_K284023 - EYFP regulated by constitutive promoter Adh1
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''Parts:''
''Parts:''
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* Lysozyme from Gallus gallus
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* BBa_K284001 - Lysozyme from Gallus gallus
''Devices to Lysozyme  characterization:''
''Devices to Lysozyme  characterization:''
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* Lysozyme constitutive expression
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* BBa_K284016 - Lysozyme constitutive expression
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* Lysozyme expression in response to lactate (DLD promoter)
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* BBa_K284017 - Lysozyme expression in response to lactate (DLD promoter)
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* Lysozyme expression in response to lactate (JEN1 promoter)
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* BBa_K284015 - Lysozyme expression in response to lactate (JEN1 promoter)

Revision as of 01:45, 22 October 2009

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The Yeastguard: Results

We confirm the following Biobricks:

Mechanism of recognition

Parts:

  • BBa_K284002 - JEN1 Promoter from Kluyveromyces lactis -
  • BBa_K284003 - Partial DLD Promoter from Kluyveromyces lactis


Devices to JEN1 and DLD promoters characterization:

  • BBa_K284020 - EYFP regulated by lactate responsive promoter (JEN1)
  • BBa_K284021 - EYFP regulated by lactate responsive promoter (DLD)
  • BBa_K284023 - EYFP regulated by constitutive promoter Adh1


Killing mechanism

Parts:

  • BBa_K284001 - Lysozyme from Gallus gallus


Devices to Lysozyme characterization:

  • BBa_K284016 - Lysozyme constitutive expression
  • BBa_K284017 - Lysozyme expression in response to lactate (DLD promoter)
  • BBa_K284015 - Lysozyme expression in response to lactate (JEN1 promoter)




Yeast experiments: Lysozyme

Growth curve



Lactococcus lactis



SDS-PAGE

We made two SDS-PAGE gels, one for the supernatant and the other for the total yeast extract. We couldn't load the pellet ethanol samples maybe because of residual ethanol at the pellets; the alcohol hinder the entry of the sample in the well.

Unfortunately we couldn't see any protein band in the gel neither in the supernatant one nor in the total extract one. Maybe we loaded insufficient amounts of sample.




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