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EPF-Lausanne/12 August 2009
From 2009.igem.org
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Wet Lab
The plates from yesterday's transformation had A LOT of clones so we did a colony PCR of about 15 clones per plate to check the efficiency of the ligation.
Redid a normal PCR on the 1.5-step PCR products since concentrations weren't high enough.
Prepared LB-Agar plates with ampicillin and kanamycin antibiotics.
Prepared a tetracycline stock (to use as an inducer). Cells were left to grow in LB medium with different concentrations of this stock at 37°C as a test for their growth speed: OD checked every hour.
We did a ligation of our read out system 2 during 1h and we did a transformation with the obtained products. Plates were left overnight at 37°C.
People in the lab
Nath, Basile, Gab, Christian, Nicolas
