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finO and finP Purification
Once we confirmed the isolation of both finO and finP sequences from the R plasmid, we now proceed to the purification of the PCR's product. It's quite important to purify the reaction since it will be used for digestion and later ligation.
We purified both PCR's products by running an agarose gel of the entire product and then extracting the desirable band from the gel. We used Invitrogen's PureLink Quick Gel Extraction Kit to perform the purification, following the manufacturer's protocol without modifications (Protocol 7).
After the procedure, we ran an agarose gel with the resulting product in order to confirm purification. We purified both finO and finP!
- Promoter of JEN1 from K. lactis
- Promoter of DLD1 from K. lactis
- Biofusion vector
- Digestions that didnt work:
- Gene JEN1 from K. lactis
- Lysozyme from G. gallus