Team:UNICAMP-Brazil/Notebooks/October 10
From 2009.igem.org
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==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
- | ====New | + | ====[https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/pGEMStrategy New Strategy: pGEM]==== |
*<p style=”text-align:justify;”>The plate of ''E. coli'' transformed with the pDLD+Biofusion had only two colonies. We did the colony PCR to find correct constructions of the pDLD and Lysozyme in Biofusion. We found two fragments corresponding to pDLD, but the negative control amplified the same fragment too! We decided to do miniprep of both colonies and re-confirm it by digestion. | *<p style=”text-align:justify;”>The plate of ''E. coli'' transformed with the pDLD+Biofusion had only two colonies. We did the colony PCR to find correct constructions of the pDLD and Lysozyme in Biofusion. We found two fragments corresponding to pDLD, but the negative control amplified the same fragment too! We decided to do miniprep of both colonies and re-confirm it by digestion. | ||
Revision as of 21:33, 21 October 2009
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