Team:PKU Beijing/Project/AND Gate 1/AND Gate Result
From 2009.igem.org
(→AND Gates of Shan Shen) |
(→AND Gates of Shan Shen) |
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==='''AND Gates of Shan Shen'''=== | ==='''AND Gates of Shan Shen'''=== | ||
- | This AND Gate family is made of luxP-supD & | + | This AND Gate family is made of luxP-supD & pLac-T7ptag. |
The typical pattern of this AND gate is like this: (picture from K228119) | The typical pattern of this AND gate is like this: (picture from K228119) | ||
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<partinfo>BBa_K228119 SequenceAndFeatures</partinfo> | <partinfo>BBa_K228119 SequenceAndFeatures</partinfo> | ||
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- | This family is the AND gate family constructed the fastest by us, it had made us much more confident about other AND gates which were under construction at that time. | + | This family is the AND gate family constructed the fastest by us, and it had made us much more confident about other AND gates which were under construction at that time. |
This AND gates family contents nine AND gates with the difference on the RBS of the T7ptag: K228119, K228120, K228121, K228122, K228123, K228124, K228125, K228126, and K228127. And these gates are on the plasmid pSB4K5. | This AND gates family contents nine AND gates with the difference on the RBS of the T7ptag: K228119, K228120, K228121, K228122, K228123, K228124, K228125, K228126, and K228127. And these gates are on the plasmid pSB4K5. | ||
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Using the E.coli with the reporter plasmid, she transformed the AND gate plasmids into it, and tested them using various combinations of different concentration of IPTG and HSL (homoserine lactone). The result is tested by a microplate reader (Fig 1, 2, 3 and 4. For more detail, refer to our [[Team:PKU_Beijing/Notebook/Protocol/Chemical_Inducible_GFP|protocol]]) | Using the E.coli with the reporter plasmid, she transformed the AND gate plasmids into it, and tested them using various combinations of different concentration of IPTG and HSL (homoserine lactone). The result is tested by a microplate reader (Fig 1, 2, 3 and 4. For more detail, refer to our [[Team:PKU_Beijing/Notebook/Protocol/Chemical_Inducible_GFP|protocol]]) | ||
- | [[Image:PKU_SS_1J.png|600px|center|thumb|Fig1. GFP expression of AND gate, K228125: lux-SupD-lac-T7ptag (rbs: J44001). The data are showed for (left to right): 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 1×10^-4 and 1×10^-3M | + | [[Image:PKU_SS_1J.png|600px|center|thumb|Fig1. GFP expression of AND gate, K228125: lux-SupD-lac-T7ptag (rbs: J44001). The data are showed for (left to right): 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 1×10^-4 and 1×10^-3M IPTG, and (bottom to top) 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 and 1×10^-4M HSL.]] |
- | [[Image:PKU_SS_11N.png|200px|left|thumb|Fig2. GFP expression of AND gate, K228124: lux-SupD-lac-T7ptag (rbs: J61127). The data are showed for (left to right): 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 1×10^-4 and 1×10^-3M | + | [[Image:PKU_SS_11N.png|200px|left|thumb|Fig2. GFP expression of AND gate, K228124: lux-SupD-lac-T7ptag (rbs: J61127). The data are showed for (left to right): 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 1×10^-4 and 1×10^-3M IPTG, and (bottom to top) 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 and 1×10^-4M HSL.]] |
- | [[Image:PKU_SS_2I.png|200px|left|thumb|Fig3. GFP expression of AND gate, K228120: lux-SupD-lac-T7ptag (rbs: B0032). The data are showed for (left to right): 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 1×10^-4 and 1×10^-3M | + | [[Image:PKU_SS_2I.png|200px|left|thumb|Fig3. GFP expression of AND gate, K228120: lux-SupD-lac-T7ptag (rbs: B0032). The data are showed for (left to right): 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 1×10^-4 and 1×10^-3M IPTG, and (bottom to top) 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 and 1×10^-4M HSL.]] |
- | [[Image:PKU_SS_2K.png|200px|left|thumb|Fig4. GFP expression of AND gate, K228126 : lux-SupD-lac-T7ptag (rbs: B0033). The data are showed for (left to right): 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 1×10^-4 and 1×10^-3M | + | [[Image:PKU_SS_2K.png|200px|left|thumb|Fig4. GFP expression of AND gate, K228126 : lux-SupD-lac-T7ptag (rbs: B0033). The data are showed for (left to right): 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 1×10^-4 and 1×10^-3M IPTG, and (bottom to top) 1×10^-9, 1×10^-8, 1×10^-7, 1×10^-6, 1×10^-5 and 1×10^-4M HSL.]] |
Discussion: | Discussion: |
Revision as of 06:55, 16 October 2009
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