Team:TUDelft/14 August 2009

From 2009.igem.org

(Difference between revisions)
(→14 August 2009)
Line 42: Line 42:
|}
|}
 +
===Calin===
 +
 +
oriTR_KO +L-ara +PCR and trbK_KO +L-ara +PCR backup plates made on 0.5x KAN.
 +
 +
Did miniprep on CB and CC culture tubes.
 +
 +
Plate 2, 4, 6, and 8 are overgrown. Cells survived electroporation. No colonies on knockout plates or control plates.
 +
 +
 +
{| border="1" align="center"
 +
| Part || DNA concentration || 260/280 || 260/230
 +
|- align="center"
 +
| CB || 57.7 || 2.17 || 2.30
 +
|- align="center"
 +
| CC || 121.1 || 2.01 || 2.33
 +
|}
 +
 +
Ran 1.5% gel with CC and CB.
 +
 +
{| border="1" align="center"
 +
| Well || Part || Expected Plasmid Size || Status
 +
|- align="center"
 +
| 1 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] || 2953 ||
 +
|- align="center"
 +
| 2 || CB || 1162 || <font color=limegreen>&#10004;</font>
 +
|- align="center"
 +
| 3 || CC || 311 || <font color=limegreen>&#10004;</font>
 +
|}
 +
 +
Digested CB with X + P (11uL DNA).
 +
Digested CC with E + S (4.5uL DNA).
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 12:58, 14 August 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

14 August 2009

Tim Weenink

First did ligation of !E and !F with the parts that were not excised from gel.

Component for !E volume (uL)
*S PCR (upstream) 2
 !C PCR (downstream) 2
pSB1AK3 from !A assembly 2
T4 ligase buffer 2
T4 ligase 1
H2O 11
Component for !F volume (uL)
 !B PCR (upstream) 2
 !D PCR (downstream) 2
pSB1AK3 from !A assembly 2
T4 ligase buffer 2
T4 ligase 1
H2O 11

Calin

oriTR_KO +L-ara +PCR and trbK_KO +L-ara +PCR backup plates made on 0.5x KAN.

Did miniprep on CB and CC culture tubes.

Plate 2, 4, 6, and 8 are overgrown. Cells survived electroporation. No colonies on knockout plates or control plates.


Part DNA concentration 260/280 260/230
CB 57.7 2.17 2.30
CC 121.1 2.01 2.33

Ran 1.5% gel with CC and CB.

Well Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] 2953
2 CB 1162 ✔
3 CC 311 ✔

Digested CB with X + P (11uL DNA). Digested CC with E + S (4.5uL DNA).


Retrieved from "http://2009.igem.org/Team:TUDelft/14_August_2009"