Team:TUDelft/25 August 2009

From 2009.igem.org

Lab Notebook

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August
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25 August 2009

Sriram

Today I got colonies in all plates for the first stage assemblies for riboregulator. I cultured colonies from plates for doing miniprep and next stage of the assembly.

Daniel

Assemblies 1B, 2B and 3B. Digestion of 4A, 2A and 1A

Gel250809.jpg

Rehydratation of oligos from BASECLEAR, PCR and purification.

Part [DNA] ng/uL
Lock for weak RBS 100.8
Key for weak RBS 128.6
Lock for medium RBS 83.1
Key for medium RBS 112.5