Team:TUDelft/17 August 2009
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+ | ===Calin=== | ||
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+ | The backup trbK_KO pate has 11 colonies. The Plate 3L oriTR_KO -L-ara +PCR also had 8 colonies. No growth on any other plates. Plates with growth put in fridge. | ||
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+ | Ran colony PCR on 5 Colonies: | ||
+ | Did colony PCR for 2 different trbK_KO colonies and also made 5mL culture tubes for them. Also did colony PCR on the oriTR control and 1 R751+pKD46+trbK_mod_S and 1 R751+pKD46+oriTR_mod_S for reference. | ||
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+ | Made 100uM and 40uM stock of verification primers. | ||
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+ | Made 1 L LB agar. | ||
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+ | Made 10mL of TRIMETHOPRIM stock. | ||
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+ | Tim Vos helped me transform CA into R751 Electrocompetent cells. Both plate and 5mL cultures made. | ||
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+ | Ran a 1% and a 2% gel with all 5 colony PCR samples. | ||
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+ | Daniel made 10 TRI, 4 CAM, and 6 AMP plates. | ||
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+ | I made a further 15 AMP + CAM plates. | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} |
Revision as of 17:40, 17 August 2009
Lab Notebook
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17 August 2009
Calin
The backup trbK_KO pate has 11 colonies. The Plate 3L oriTR_KO -L-ara +PCR also had 8 colonies. No growth on any other plates. Plates with growth put in fridge.
Ran colony PCR on 5 Colonies: Did colony PCR for 2 different trbK_KO colonies and also made 5mL culture tubes for them. Also did colony PCR on the oriTR control and 1 R751+pKD46+trbK_mod_S and 1 R751+pKD46+oriTR_mod_S for reference.
Made 100uM and 40uM stock of verification primers.
Made 1 L LB agar.
Made 10mL of TRIMETHOPRIM stock.
Tim Vos helped me transform CA into R751 Electrocompetent cells. Both plate and 5mL cultures made.
Ran a 1% and a 2% gel with all 5 colony PCR samples.
Daniel made 10 TRI, 4 CAM, and 6 AMP plates.
I made a further 15 AMP + CAM plates.