Team:TUDelft/31 July 2009

From 2009.igem.org

Revision as of 18:55, 31 July 2009 by Cplesa (Talk | contribs)

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

31 July 2009

Tim Weenink

Did some minipreps today. Then nanodropped them:

Biobrick name Internal reference Concentration ng/µl 260/280 260/230
BBa_K142205 *S2 86.4 2.01 2.03
BBa_K142205 *S3 107.7 2.03 2.11
BBa_K142205 *S4 69.0 1.97 1.96
BBa_K142205 *S5 87.4 1.98 1.99
BBa_K142202 *T1 128.5 1.99 2.09
BBa_K142202 *T2 129.4 2.02 2.16
BBa_K142202 *T3 126.6 1.97 2.06
BBa_K142202 *T4 106.7 1.95 2.05
N/A  !A 171.2 1.98 2.11

Calin

Cultures in all pSB4C5 tubes. Did miniprep on all tubes and also made a plate.

Part Concentration ng/µl 260/280 260/230
pSB4C5 I 123.0 1.91 2.06
pSB4C5 II 125.8 1.92 2.29
pSB4C5 III 130.7 1.95 2.07

Todays gel confirmed that oriT-R digestion failed and pSB4C5 is way too large.

Gel31072009.png


Well Part Expected Plasmid Size Status
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
23 oriT E + P digest 2079
24 pSB4C5 I 3896
25 pSB4C5 II 3896
26 pSB4C5 III 3896

R751 cells arrived. Made two plates without antibiotics.

Digestions were started:

position parts volume in µl
Upstream BBa_I13522 pTet-GFP 27.0
H2O 15.5
Upstream BBa_E0840 rbs-GFP-term 5.0
H2O 37.5
Backbone pSB1C3 5.0
H2O 37.5