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Team:TUDelft/4 August 2009
From 2009.igem.org
Lab Notebook
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4 August 2009
Orr
Prepared some LB Agar medium, and continued with writing the code for the software constructing lock and key sequences for the cascade delay device.
Calin
Trimethoprim finally arrived. Made trimethoprim 4 x 1 mL eppendorfs stock solution of 10 mg/mL using DMSO solution.
Transformed the first two ligations (CA & CB).
Made two R751 plates with 2xTRI.
Made a 5 mL tube of pTet-GFP with AMP and another 5 mL tube of R751 with TRI.
