Team:Cambridge/Notebook/Week7

From 2009.igem.org

Revision as of 20:08, 24 August 2009 by Vmullin (Talk | contribs)


Week 7

Monday

Wet Work

Amplification

Colony PCR of 8 colonies picked from 391 in pSB3K3 transformants, 8 colonies picked from 374 in pSB3K3 transformants, and 1 colony picked from 381 in pSB3K3 transformants, overnight cultures of each of those colonies as well.

MelA BioBrick

PCR done of the origonal MelA and the MelA inbtermediate to biobrick (without the second PstI site). This was too create enough DNA for a resitriction digest to be carried out to check that the previous weeks PCRs had been sucessful. After PCR, the products were run on a gel and extracted.

We also tested the Primer C again, as it didn't work last time. Three PCR's were done:

  • Primer F and Primer C used last time
  • Primer F and a new aliquot of Primer C
  • Primer F and revortexed and re-aliquoted primer C from the origonal vial

Dry Work

We found the phzM and phzS pigments in the registry!

  • BBa_I723024 (phzM)
  • BBa_I723025 (phzS)

This means we can now start working on these pigments, connecting them to processing and logic systems.

Cambridge Sponsor Logo1.pngCambridge Sponsor Logo2.pngCambridge Sponsor Logo3.pngCambridge Sponsor Logo4.pngCambridge Sponsor Logo5.pngCambridge Sponsor Logo8.pngCambridge Sponsor Logo6.pngCambridge Sponsor Logo7.pngCambridge Sponsor Logo9.pngCambridge Sponsor Logo10.pngCambridge Sponsor Logo11.pngCambridge Sponsor Logo12.pngCambridge Sponsor Logo14.pngCambridge Sponsor Logo13.pngCambridge Sponsor Logo15.pngCambridge Sponsor Logo16.pngCambridge Sponsor Logo17.pngCambridge Sponsor Logo18.pngCambridge Sponsor Logo19.pngBmglab.jpg