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Team:Cambridge/Project/Amplification
From 2009.igem.org
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The Cambridge 2007 iGEM team developed a Pops amplifier system using phage activators and promoters. The system works by using a Pops input to make an activator protein, as shown in the diagram from their wiki below, which then binds to a promoter and generates a Pops output. | The Cambridge 2007 iGEM team developed a Pops amplifier system using phage activators and promoters. The system works by using a Pops input to make an activator protein, as shown in the diagram from their wiki below, which then binds to a promoter and generates a Pops output. | ||
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[[Image:amplifier07.jpg]] | [[Image:amplifier07.jpg]] | ||
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| + | In order to quantify the ratio between Pops in and Pops out, the team built the following constructions, with mRFP and GFP as Pops reporters. | ||
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| + | [[Image:construction07.jpg]] | ||
== Project Details == | == Project Details == | ||
Revision as of 09:13, 4 August 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
A Reliable Amplification System
Abstract
The Cambridge 2007 iGEM team developed a Pops amplifier system using phage activators and promoters. The system works by using a Pops input to make an activator protein, as shown in the diagram from their wiki below, which then binds to a promoter and generates a Pops output.
In order to quantify the ratio between Pops in and Pops out, the team built the following constructions, with mRFP and GFP as Pops reporters.
