Team:Cambridge/Project/Violacein

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Violacein Pigment

Introduction

The Violacein pigment is produced from L-tryptophan via an pathway involving four enzymes, vioA-D. This scheme is shown below:

Violacein pigment production.jpg

As module 5 is Aqua, this gives us two different colour controlled by the four genes allowing the construction of logic gates.

Research by previous iGem teams

Previous biobricks:

Registry Code Team Sequence Description Notes

Action plan of our team

Our action plan is as follows:

1.

Test activity of X

Mapping of Biobrick X: (image)

Construct composite Biobrick X

Plan mapping of X:







(image)

Test colour changes by X

Characterisation of Biobricks and colour output

Test compatibility with other biobricks