Team:Edinburgh/biology(materialsandmethods)

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       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28globetutorial%29">Globe Tutorial</a></li>
       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28globetutorial%29">Globe Tutorial</a></li>
       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28igemwikhacks%29">iGEM WIKI Hacks</a></li>
       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28igemwikhacks%29">iGEM WIKI Hacks</a></li>
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       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28conclusions%29">Conclusions</a></li>
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       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28conclusions%29">Blog Entry</a></li>
     </ul>
     </ul>
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<th><center>Protocol Name</center></th>
<th><center>Protocol Name</center></th>
<th><center>Download Link</center></th>
<th><center>Download Link</center></th>
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                                <th><center>Comments</center></th>
 
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<td><center>Creating Composite BioBrick</center></td>
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                        <tr>
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<td><center></center></td>
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<td><center>Making Competent Cells</center></td>
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                                <td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/1/19/1._Making_Competent_Cells.zip">Download</a></center></td>
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<td><center>Transformation of Cells</center></td>
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<td><center>Transforming <i>E. coli</i> cells by heat shock</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/b/be/2._Transforming_E._coli_cells_by_heat_shock.zip">Download</a></center></td>
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                                <td><center></center></td>
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<td><center>Cloning BioBrick Parts by Fusion PRC</center></td>
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<td><center>Transforming <i>E. coli</i> cells by CaC12</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/c/cc/3._Transforming_E._coli_cells_by_CaCl2.zip">Download</a></center></td>
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<td><center>Protocol to Store Cell Cultures</center></td>
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<td><center>Transducing of <i>E. coli</i> cells</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/d/d7/5._Transduction_of_E._Coli.zip">Download</a></center></td>
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                                <td><center></center></td>
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<td><center>Removal of PstI Site on onr Enzyme</center></td>
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<td><center>Making P1 lysate</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/c/cb/6._Making_P1_lysate.zip">Download</a></center></td>
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                                <td><center></center></td>
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<td><center>Sequencing Protocol</center></td>
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<td><center>Storing Cell Cultures</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/7/77/7._Storing_Cell_Cultures.zip">Download</a></center></td>
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                                <td><center></center></td>
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<td><center>Competent Cells Preparation</center></td>
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<td><center>PCR using Kod Polymerase</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/1/12/8._PCR_using_Kod_Polymearse.zip">Download</a></center></td>
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                                <td><center></center></td>
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<td><center>Transduction of <i>E.Coli</i></center></td>
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<td><center>Fusion PCR</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/b/ba/9._Fusion_PCR.zip">Download</a></center></td>
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                                <td><center></center></td>
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<td><center>Making of P1 Lysate</center></td>
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<td><center>Sequencing protocol</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/0/03/10._Sequencing_Protocol.zip">Download</a></center></td>
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<td><center>Quick <i>E.coli</i> transformations using CaCl2</center></td>
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<td><center>Building Composite BioBrick Parts</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/7/71/11._Building_composite_biobrick_parts.zip">Download</a></center></td>
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                                <td><center></center></td>
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<td><center>PCR</center></td>
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<td><center></center></td>
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</table>
</table>
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<th><center>Primers</center></th>
<th><center>Primers</center></th>
<th><center>Download Link</center></th>
<th><center>Download Link</center></th>
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                                <th><center>Comments</center></th>
 
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<td><center><i>E.Coli</i> Nitrate-Nitrite Sensing Upstream Primers</center></td>
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<td><center><i>TNT receptors 1 and 3</i></center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/8/8c/1._TNT_receptors_1_and_3.zip">Download</a></center></td>
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                                <td><center></center></td>
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<td><center><i>Trz</i> fusion protein</center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/a/a0/2._Trz_fusion_protein.zip">Download</a></center></td>
</tr>
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<td><center>Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase</center></td>
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<td><center><i>yeaR</i> and <i>ytfE</i> upstream region</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/c/c8/3._yeaR_and_ytfE_upstream_region.zip">Download</a></center></td>
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                                <td><center></center></td>
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<td><center>Lux Operon Primers</center></td>
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<td><center><i>Photobacterium Phosphoreum lux operon</i></center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/2/22/4._Photobacterium_Phosphoreum_Lux_operon.zip">Download</a></center></td>
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                                <td><center></center></td>
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<td><center>Pentaerythritol Tetranitrate Reductase Primers</center></td>
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<td><center><i>Photobacterium Phosphoreum</i>lumP</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/5/5a/5._photobacterium_phosphoreum_lumazine_protein_%28lumP%29.zip">Download</a></center></td>
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                                <td><center></center></td>
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</tr>
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<td><center>TNT.R1 A Receptor With Fair Affinity</center></td>
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<td><center>ptrp</center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/9/93/Ptrp.zip">Download</a></center></td>
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                                <td><center></center></td>
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<tr>
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<td><center>TrzI Primers</center></td>
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<td><center>nsrR and nir promoter from <i>N. Europaea</i></center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/0/02/NsrR_and_nir_promoter_from_N._Europaea.zip">Download</a></center></td>
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                                <td><center></center></td>
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</tr>
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<td><center>Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase (onr) Gene</center></td>
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<td><center><i>Xenorhabdus luminescens luciferase</i></center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/2/29/Primer-_Xenorhabdus_luminescens_luciferase.zip">Download</a></center></td>
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                                <td><center></center></td>
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</tr>
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<td><center><i>Photobacterium Phosphoreum</i> Lumazine Protein (lumP) Gene</center></td>
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<td><center><i>PETN reductase <i>(onr)</i></center></td>
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<td><center></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/9/90/Primer_-_PETN_reductase_%28onr%29.zip">Download</a></center></td>
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                                <td><center></center></td>
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</tr>
</tr>

Latest revision as of 23:03, 21 October 2009

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Biology - Material and Methods
Protocol Name
Download Link
Making Competent Cells
Download
Transforming E. coli cells by heat shock
Download
Transforming E. coli cells by CaC12
Download
Transducing of E. coli cells
Download
Making P1 lysate
Download
Storing Cell Cultures
Download
PCR using Kod Polymerase
Download
Fusion PCR
Download
Sequencing protocol
Download
Building Composite BioBrick Parts
Download
Primers
Download Link
TNT receptors 1 and 3
Download
Trz fusion protein
Download
yeaR and ytfE upstream region
Download
Photobacterium Phosphoreum lux operon
Download
Photobacterium PhosphoreumlumP
Download
ptrp
Download
nsrR and nir promoter from N. Europaea
Download
Xenorhabdus luminescens luciferase
Download
PETN reductase (onr)
Download
Edinburgh University iGEM Team 2009

Retrieved from "http://2009.igem.org/Team:Edinburgh/biology(materialsandmethods)"