Team:Edinburgh/biology(materialsandmethods)

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Latest revision as of 23:03, 21 October 2009

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Overall Description & Design

TNT-Sensing Pathway

Nitrite/Nitrate-Sensing Pathway

Biobrick Parts

Results

Problem Solving and Tips

Materials and Methods

References

Biology - Material and Methods

Protocol Name	Download Link
Making Competent Cells	Download
Transforming E. coli cells by heat shock	Download
Transforming E. coli cells by CaC12	Download
Transducing of E. coli cells	Download
Making P1 lysate	Download
Storing Cell Cultures	Download
PCR using Kod Polymerase	Download
Fusion PCR	Download
Sequencing protocol	Download
Building Composite BioBrick Parts	Download

Primers	Download Link
TNT receptors 1 and 3	Download
Trz fusion protein	Download
yeaR and ytfE upstream region	Download
Photobacterium Phosphoreum lux operon	Download
Photobacterium PhosphoreumlumP	Download
ptrp	Download
nsrR and nir promoter from N. Europaea	Download
Xenorhabdus luminescens luciferase	Download
PETN reductase (onr)	Download

Edinburgh University iGEM Team 2009

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+                  <div id="Upgradebox">
+	            <div id="Upgradenote" align="left">
+                      Our WIKI is best viewed with (click title):<br /><br />
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+                      If you encounter any visual problems please upgrade your browser to the latest version in order to get the most out of our WIKI. Thank you!
+                    </div>
+                  </div>
 		</div>
 		<div id=mainmenu>
 			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28overalldescription%29"> Overall Description & Design </a> </div>
-			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28tntsensing%29"> TNT-Sensing </a> </div>
+			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28tntsensing%29"> TNT-Sensing Pathway</a> </div>
-			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28nitritenitratesensing%29"> Nitrite/Nitrate-Sensing </a> </div>
+			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28nitritenitratesensing%29"> Nitrite/Nitrate-Sensing Pathway</a> </div>
+                        <div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28biobricks%29"> Biobrick Parts </a> </div>
+			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28results%29"> Results </a> </div>
+			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28solvedproblems%29"> Problem Solving and Tips </a> </div>
 			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28materialsandmethods%29"> Materials and Methods </a> </div>
-			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28biobricks%29"> Biobricks </a> </div>
-			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28solvedproblems%29"> Problem Solving and Tips </a> </div>
-			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28results%29"> Results </a> </div>
-			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28glanceatthefuture%29"> Future Work </a> </div>
 			<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28references%29"> References </a> </div>
 		</div>
@@ Line 204: / Line 229: @@
     <ul>
      <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28overalldescription%29">Overall Description and Design</a></li>
-     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28tntsensing%29">TNT-Sensing</a></li>
+     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28tntsensing%29">TNT-Sensing Pathway</a></li>
-     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28nitritenitratesensing%29">Nitrite/Nitrate-Sensing</a></li>
+     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28nitritenitratesensing%29">Nitrite/Nitrate-Sensing Pathway</a></li>
-    <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28materialsandmethods%29">Materials and Methods</a></li>
+     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28biobricks%29">Biobrick Parts</a></li>
-     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28biobricks%29">Biobricks</a></li>
-    <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28solvedproblems%29">Problem Solving and Tips</a></li>
      <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28results%29">Results</a></li>
-     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28glanceatthefuture%29">Future Work</a></li>
+     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28solvedproblems%29">Problem Solving and Tips</a></li>
+    <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28materialsandmethods%29">Materials and Methods</a></li>
      <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28references%29">References</a></li>
     </ul>
@@ Line 226: / Line 250: @@
    </li>
-   <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28introduction%29" class="dir">Underlying Philosophy</a>
+   <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28publicperception%29" class="dir">Underlying Philosophy</a>
      <ul>
       <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28publicperception%29">Public Perception</a></li>
       <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28legislationissues%29">Legislation issues</a></li>
       <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28biosafety%29">Biosafety</a></li>
-      <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28religiousperception%29">Religious Perception</a></li>
+      <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28summary%29">DEMOCS Card Game</a></li>
      </ul>
     </li>
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       <ul>
        <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28introduction%29">Introduction</a></li>
-      <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28conceptsandtechnology%29">Concepts and Technologies</a></li>
        <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28globetutorial%29">Globe Tutorial</a></li>
        <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28igemwikhacks%29">iGEM WIKI Hacks</a></li>
-       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28conclusions%29">Conclusions</a></li>
+       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28conclusions%29">Blog Entry</a></li>
       </ul>
    </li>
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    <li><a href="https://2009.igem.org/Team:Edinburgh/Notebook">Notebook</a></li>
-   <li><a href="https://2009.igem.org/Team:Edinburgh/team%28teamintroduction%29" class="dir">Team</a>
+   <li><a href="https://2009.igem.org/Team:Edinburgh/team%28teammembers%29" class="dir">Team</a>
     <ul>
 	 <li><a href="https://2009.igem.org/Team:Edinburgh/team%28teammembers%29">Team Members</a></li>
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 <img src="https://static.igem.org/mediawiki/2009/6/68/EdinburghPagemarker.jpg" style="margin-left:766px;">
-<div id="abstarct" style="background-color:#e4f5ca;border:1px solid #595151;width:755px;height:220px;margin-top:10px;margin-left:20px;font-size:11px;text-align:justify;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;">
+<img src="https://static.igem.org/mediawiki/2009/7/7a/ProtocolsPrimers.jpg" style="margin-left:-5px;margin-top:0px;">
-<b> Personal note </b> <br /><br />
-<font color="green" style="float:right">CF</font>
+<div id="text" style="margin-left:20px;margin-top:10px;padding-bottom:15px;">
-</div>
+<table cellspacing="0" cellpadding="0">
+			<tr>
+				<th><center>Protocol Name</center></th>
+				<th><center>Download Link</center></th>
+			</tr>
+                        <tr>
+				<td><center>Making Competent Cells</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/1/19/1._Making_Competent_Cells.zip">Download</a></center></td>
+			</tr>
+                        <tr>
+				<td><center>Transforming <i>E. coli</i> cells by heat shock</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/b/be/2._Transforming_E._coli_cells_by_heat_shock.zip">Download</a></center></td>
+			</tr>
+                        <tr>
+				<td><center>Transforming <i>E. coli</i> cells by CaC12</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/c/cc/3._Transforming_E._coli_cells_by_CaCl2.zip">Download</a></center></td>
+			</tr>
+                        <tr>
+				<td><center>Transducing of <i>E. coli</i> cells</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/d/d7/5._Transduction_of_E._Coli.zip">Download</a></center></td>
+			</tr>
+                        <tr>
+				<td><center>Making P1 lysate</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/c/cb/6._Making_P1_lysate.zip">Download</a></center></td>
+			</tr>
+                        <tr>
+				<td><center>Storing Cell Cultures</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/7/77/7._Storing_Cell_Cultures.zip">Download</a></center></td>
+			</tr>
+                        <tr>
+				<td><center>PCR using Kod Polymerase</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/1/12/8._PCR_using_Kod_Polymearse.zip">Download</a></center></td>
+			</tr>
+                        <tr>
+				<td><center>Fusion PCR</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/b/ba/9._Fusion_PCR.zip">Download</a></center></td>
+			</tr>
+                        <tr>
+				<td><center>Sequencing protocol</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/0/03/10._Sequencing_Protocol.zip">Download</a></center></td>
+			</tr>
+                        <tr>
+				<td><center>Building Composite BioBrick Parts</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/7/71/11._Building_composite_biobrick_parts.zip">Download</a></center></td>
+			</tr>
+</table>
-<img src="https://static.igem.org/mediawiki/2009/7/7a/ProtocolsPrimers.jpg" style="margin-left:-5px;margin-top:20px;">
+<table cellspacing="0" cellpadding="0">
+			<tr>
+				<th><center>Primers</center></th>
+				<th><center>Download Link</center></th>
+			</tr>
+			<tr>
+				<td><center><i>TNT receptors 1 and 3</i></center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/8/8c/1._TNT_receptors_1_and_3.zip">Download</a></center></td>
-<div id="text" style="margin-left:20px;margin-top:10px;padding-bottom:15px;">
+			</tr>
+			<tr>
+				<td><center><i>Trz</i> fusion protein</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/a/a0/2._Trz_fusion_protein.zip">Download</a></center></td>
-</div>
+			</tr>
+			<tr>
+				<td><center><i>yeaR</i> and <i>ytfE</i> upstream region</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/c/c8/3._yeaR_and_ytfE_upstream_region.zip">Download</a></center></td>
-	<div id=bottomlinks align=right>
+			</tr>
-		<div id=link><a href=#>next page</a></div>
+			<tr>
-		<div id=link><a href=#>previous page</a></div>
+				<td><center><i>Photobacterium Phosphoreum lux operon</i></center></td>
-	</div>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/2/22/4._Photobacterium_Phosphoreum_Lux_operon.zip">Download</a></center></td>
+			</tr>
+			<tr>
+				<td><center><i>Photobacterium Phosphoreum</i>lumP</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/5/5a/5._photobacterium_phosphoreum_lumazine_protein_%28lumP%29.zip">Download</a></center></td>
+			</tr>
+			<tr>
+				<td><center>ptrp</center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/9/93/Ptrp.zip">Download</a></center></td>
+			</tr>
+			<tr>
+				<td><center>nsrR and nir promoter from <i>N. Europaea</i></center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/0/02/NsrR_and_nir_promoter_from_N._Europaea.zip">Download</a></center></td>
+			</tr>
+			<tr>
+				<td><center><i>Xenorhabdus luminescens luciferase</i></center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/2/29/Primer-_Xenorhabdus_luminescens_luciferase.zip">Download</a></center></td>
+			</tr>
+			<tr>
+				<td><center><i>PETN reductase <i>(onr)</i></center></td>
+				<td><center><a href="https://static.igem.org/mediawiki/2009/9/90/Primer_-_PETN_reductase_%28onr%29.zip">Download</a></center></td>
+			</tr>
+</table>
+</div>
 			</div>
 			<div id=bottombox>