Team:Edinburgh/biology(materialsandmethods)

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                      Our WIKI is best viewed with (click title):<br /><br />
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<div id=mainmenu>
<div id=mainmenu>
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<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28overalldescription%29"> Overall Description & Design </a> </div>   
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<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28overalldescription%29"> Overall Description & Design </a> </div>
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<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28tntsensing%29"> TNT-Sensing Pathway</a> </div>
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<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28nitritenitratesensing%29"> Nitrite/Nitrate-Sensing Pathway</a> </div>
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                        <div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28biobricks%29"> Biobrick Parts </a> </div>
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<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28results%29"> Results </a> </div>
 +
<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28solvedproblems%29"> Problem Solving and Tips </a> </div>
<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28materialsandmethods%29"> Materials and Methods </a> </div>
<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28materialsandmethods%29"> Materials and Methods </a> </div>
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<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28biobricks%29"> Biobricks </a> </div>
 
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<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28solvedproblems%29"> Problem Solving and Tips </a> </div>
 
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<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28results%29"> Results </a> </div>
 
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<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28glanceatthefuture%29"> Future Work </a> </div>
 
<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28references%29"> References </a> </div>
<div id=menuitem >  <a href="https://2009.igem.org/Team:Edinburgh/biology%28references%29"> References </a> </div>
</div>
</div>
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<ul id="nav" class="dropdown dropdown-horizontal">
<ul id="nav" class="dropdown dropdown-horizontal">
  <li><a href="https://2009.igem.org/Team:Edinburgh">Home</a></li>
  <li><a href="https://2009.igem.org/Team:Edinburgh">Home</a></li>
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<li><a href="https://2009.igem.org/Team:Edinburgh/projectmain%28overall%29" class="dir">Top View</a>
 
-
  <ul>
 
-
  <li><a href="https://2009.igem.org/Team:Edinburgh/projectmain%28overall%29">Overall Description</a></li>
 
-
  <li><a href="https://2009.igem.org/Team:Edinburgh/projectmain%28motivationl%29">Motivation</a></li>
 
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  <li><a href="https://2009.igem.org/Team:Edinburgh/projectmain%28whywediffer%29">Why We Differ?</a></li>
 
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  <li><a href="https://2009.igem.org/Team:Edinburgh/projectmain%28glanceatthefuture%29">Glance At The Future</a></li>
 
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  <li><a href="https://2009.igem.org/Team:Edinburgh/projectmain%28references%29">References</a></li>
 
-
  </ul>
 
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  </li>
 
   <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28overalldescription%29" class="dir">Biology</a>
   <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28overalldescription%29" class="dir">Biology</a>
   <ul>
   <ul>
     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28overalldescription%29">Overall Description and Design</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28overalldescription%29">Overall Description and Design</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28materialsandmethods%29">Materials and Methods</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28tntsensing%29">TNT-Sensing Pathway</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28biobricks%29">Biobricks</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28nitritenitratesensing%29">Nitrite/Nitrate-Sensing Pathway</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28solvedproblems%29">Problem Solving and Tips</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28biobricks%29">Biobrick Parts</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28results%29">Results</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28results%29">Results</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28glanceatthefuture%29">Future Work</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28solvedproblems%29">Problem Solving and Tips</a></li>
 +
    <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28materialsandmethods%29">Materials and Methods</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28references%29">References</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/biology%28references%29">References</a></li>
   </ul>
   </ul>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/reallifeapplication%28scaleup%29">Scale Up</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/reallifeapplication%28scaleup%29">Scale Up</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/modelling%28results%29">Results</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/modelling%28results%29">Results</a></li>
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    <li><a href="https://2009.igem.org/Team:Edinburgh/modelling%28glanceatthefuture%29">Glance At the Future</a></li>
 
     <li><a href="https://2009.igem.org/Team:Edinburgh/modelling%28references%29">References</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/modelling%28references%29">References</a></li>
   </ul>
   </ul>
   </li>
   </li>
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   <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28introduction%29" class="dir">Underlying Philosophy</a>
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   <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28publicperception%29" class="dir">Underlying Philosophy</a>
     <ul>
     <ul>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28introduction%29">Introduction</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28publicperception%29">Public Perception</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28legislationissues%29">Legislation issues</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28legislationissues%29">Legislation issues</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28biosafety%29">Biosafety</a></li>
     <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28biosafety%29">Biosafety</a></li>
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    <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28religiousperception%29">Religious Perception</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28summary%29">DEMOCS Card Game</a></li>
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    <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28publicperception%29">Public Perception</a></li>
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    <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28surveyandconclusions%29">Survey and Conclusions</a></li>
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     <li><a href="https://2009.igem.org/Team:Edinburgh/ethics%28summary%29">Summary (Synthetic Bilogy Pro and Contras)</a></li>
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     </ul>
     </ul>
   </li>
   </li>
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   <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28introduction%29" class="dir">New Informatics</a>
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   <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28introduction%29" class="dir">Informatics</a>
     <ul>
     <ul>
       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28introduction%29">Introduction</a></li>
       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28introduction%29">Introduction</a></li>
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      <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28conceptsandtechnology%29">Concepts and Technologies</a></li>
 
       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28globetutorial%29">Globe Tutorial</a></li>
       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28globetutorial%29">Globe Tutorial</a></li>
       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28igemwikhacks%29">iGEM WIKI Hacks</a></li>
       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28igemwikhacks%29">iGEM WIKI Hacks</a></li>
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       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28conclusions%29">Conclusions</a></li>
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       <li><a href="https://2009.igem.org/Team:Edinburgh/newinformatics%28conclusions%29">Blog Entry</a></li>
     </ul>
     </ul>
   </li>
   </li>
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  <li><a href="https://2009.igem.org/Team:Edinburgh/results">Results</a></li>
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   <li><a href="https://2009.igem.org/Team:Edinburgh/Notebook">Notebook</a></li>
   <li><a href="https://2009.igem.org/Team:Edinburgh/Notebook">Notebook</a></li>
    
    
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   <li><a href="https://2009.igem.org/Team:Edinburgh/team%28teamintroduction%29" class="dir">Team</a>
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   <li><a href="https://2009.igem.org/Team:Edinburgh/team%28teammembers%29" class="dir">Team</a>
   <ul>
   <ul>
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<li><a href="https://2009.igem.org/Team:Edinburgh/team%28teamintroduction%29">Team Introduction</a></li>
 
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<li><a href="https://2009.igem.org/Team:Edinburgh/team%28edinburghuniversity%29">Edinburgh University</a></li>
 
<li><a href="https://2009.igem.org/Team:Edinburgh/team%28teammembers%29">Team Members</a></li>
<li><a href="https://2009.igem.org/Team:Edinburgh/team%28teammembers%29">Team Members</a></li>
<li><a href="https://2009.igem.org/Team:Edinburgh/team%28advisors%29">Advisors</a></li>
<li><a href="https://2009.igem.org/Team:Edinburgh/team%28advisors%29">Advisors</a></li>
<li><a href="https://2009.igem.org/Team:Edinburgh/team%28supervisors%29">Supervisors</a></li>
<li><a href="https://2009.igem.org/Team:Edinburgh/team%28supervisors%29">Supervisors</a></li>
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        <li><a href="https://2009.igem.org/Team:Edinburgh/team%28our message%29">Our Message</a></li>
 
         <li><a href="https://2009.igem.org/Team:Edinburgh/team%28gallery%29">Gallery</a></li>
         <li><a href="https://2009.igem.org/Team:Edinburgh/team%28gallery%29">Gallery</a></li>
         <li><a href="https://2009.igem.org/Team:Edinburgh/team%28contacts%29">Contacts</a></li>
         <li><a href="https://2009.igem.org/Team:Edinburgh/team%28contacts%29">Contacts</a></li>
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<img src="https://static.igem.org/mediawiki/2009/6/68/EdinburghPagemarker.jpg" style="margin-left:766px;">
<img src="https://static.igem.org/mediawiki/2009/6/68/EdinburghPagemarker.jpg" style="margin-left:766px;">
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<div id="abstarct" style="background-color:#e4f5ca;border:1px solid #595151;width:755px;height:220px;margin-top:10px;margin-left:20px;font-size:11px;text-align:justify;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;">
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<img src="https://static.igem.org/mediawiki/2009/7/7a/ProtocolsPrimers.jpg" style="margin-left:-5px;margin-top:0px;">
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<b> Abstract </b> <br /><br />
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<div id="text" style="margin-left:20px;margin-top:10px;padding-bottom:15px;">
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</div>
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<img src="https://static.igem.org/mediawiki/2009/3/3b/EdinburghParagraphMarker.jpg" style="margin-left:-5px;margin-top:20px;">
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<table cellspacing="0" cellpadding="0">
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<tr>
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<th><center>Protocol Name</center></th>
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<th><center>Download Link</center></th>
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</tr>
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                        <tr>
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<td><center>Making Competent Cells</center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/1/19/1._Making_Competent_Cells.zip">Download</a></center></td>
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</tr>
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                        <tr>
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<td><center>Transforming <i>E. coli</i> cells by heat shock</center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/b/be/2._Transforming_E._coli_cells_by_heat_shock.zip">Download</a></center></td>
 +
</tr>
 +
                        <tr>
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<td><center>Transforming <i>E. coli</i> cells by CaC12</center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/c/cc/3._Transforming_E._coli_cells_by_CaCl2.zip">Download</a></center></td>
 +
</tr>
 +
                        <tr>
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<td><center>Transducing of <i>E. coli</i> cells</center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/d/d7/5._Transduction_of_E._Coli.zip">Download</a></center></td>
 +
</tr>
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                        <tr>
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<td><center>Making P1 lysate</center></td>
 +
<td><center><a href="https://static.igem.org/mediawiki/2009/c/cb/6._Making_P1_lysate.zip">Download</a></center></td>
 +
</tr>
 +
                        <tr>
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<td><center>Storing Cell Cultures</center></td>
 +
<td><center><a href="https://static.igem.org/mediawiki/2009/7/77/7._Storing_Cell_Cultures.zip">Download</a></center></td>
 +
</tr>
 +
                        <tr>
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<td><center>PCR using Kod Polymerase</center></td>
 +
<td><center><a href="https://static.igem.org/mediawiki/2009/1/12/8._PCR_using_Kod_Polymearse.zip">Download</a></center></td>
 +
</tr>
 +
                        <tr>
 +
<td><center>Fusion PCR</center></td>
 +
<td><center><a href="https://static.igem.org/mediawiki/2009/b/ba/9._Fusion_PCR.zip">Download</a></center></td>
 +
</tr>
 +
                        <tr>
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<td><center>Sequencing protocol</center></td>
 +
<td><center><a href="https://static.igem.org/mediawiki/2009/0/03/10._Sequencing_Protocol.zip">Download</a></center></td>
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</tr>
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                        <tr>
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<td><center>Building Composite BioBrick Parts</center></td>
 +
<td><center><a href="https://static.igem.org/mediawiki/2009/7/71/11._Building_composite_biobrick_parts.zip">Download</a></center></td>
 +
</tr>
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 +
</table>
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<div id="text" style="margin-left:20px;margin-top:10px;padding-bottom:15px;">
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<table cellspacing="0" cellpadding="0">
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Praesent nec nisi nec elit volutpat sollicitudin. Sed cursus venenatis egestas. In mollis vehicula dictum. Sed id lacus vitae tortor commodo pharetra porttitor ut purus. Nullam euismod urna at felis accumsan sit amet viverra leo fermentum. Phasellus ac molestie sapien. In in neque purus. Cras eget lorem arcu. Aliquam ornare tellus vel nisi commodo molestie. Aliquam aliquet magna nisl. Quisque velit nulla, adipiscing ut sagittis id, luctus viverra leo. Nam nunc nisl, euismod eu dignissim nec, eleifend ut augue. Phasellus tempor condimentum ipsum, ut molestie lorem suscipit vel. Nulla luctus turpis eget neque facilisis eget sodales ante tincidunt. Praesent congue lobortis mollis. Maecenas non augue id mi dignissim luctus. Donec quis volutpat lectus. Mauris condimentum sodales luctus.
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<tr>
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<th><center>Primers</center></th>
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<th><center>Download Link</center></th>
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</tr>
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<tr>
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<td><center><i>TNT receptors 1 and 3</i></center></td>
 +
<td><center><a href="https://static.igem.org/mediawiki/2009/8/8c/1._TNT_receptors_1_and_3.zip">Download</a></center></td>
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Nulla nec mauris sapien, eget gravida quam. Fusce vulputate risus eget diam elementum vel mattis nisi sagittis. Vivamus eget enim a nisl imperdiet aliquet commodo eget turpis. Aliquam nec mi ac neque tincidunt facilisis fringilla at sapien. Praesent in justo vel turpis hendrerit suscipit. Nullam nec magna leo, sed vulputate quam. Aliquam sollicitudin hendrerit erat at eleifend. Vivamus tincidunt felis vitae metus suscipit non volutpat neque tempus. Phasellus sagittis accumsan tellus, vitae pharetra ligula rhoncus vitae.
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</tr>
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<tr>
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<td><center><i>Trz</i> fusion protein</center></td>
 +
<td><center><a href="https://static.igem.org/mediawiki/2009/a/a0/2._Trz_fusion_protein.zip">Download</a></center></td>
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<br /><br />
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</tr>
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<tr>
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<td><center><i>yeaR</i> and <i>ytfE</i> upstream region</center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/c/c8/3._yeaR_and_ytfE_upstream_region.zip">Download</a></center></td>
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Praesent nec nisi nec elit volutpat sollicitudin. Sed cursus venenatis egestas. In mollis vehicula dictum. Sed id lacus vitae tortor commodo pharetra porttitor ut purus. Nullam euismod urna at felis accumsan sit amet viverra leo fermentum. Phasellus ac molestie sapien. In in neque purus. Cras eget lorem arcu. Aliquam ornare tellus vel nisi commodo molestie. Aliquam aliquet magna nisl. Quisque velit nulla, adipiscing ut sagittis id, luctus viverra leo. Nam nunc nisl, euismod eu dignissim nec, eleifend ut augue. Phasellus tempor condimentum ipsum, ut molestie lorem suscipit vel. Nulla luctus turpis eget neque facilisis eget sodales ante tincidunt. Praesent congue lobortis mollis. Maecenas non augue id mi dignissim luctus. Donec quis volutpat lectus. Mauris condimentum sodales luctus.
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<td><center><i>Photobacterium Phosphoreum lux operon</i></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/2/22/4._Photobacterium_Phosphoreum_Lux_operon.zip">Download</a></center></td>
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Nulla nec mauris sapien, eget gravida quam. Fusce vulputate risus eget diam elementum vel mattis nisi sagittis. Vivamus eget enim a nisl imperdiet aliquet commodo eget turpis. Aliquam nec mi ac neque tincidunt facilisis fringilla at sapien. Praesent in justo vel turpis hendrerit suscipit. Nullam nec magna leo, sed vulputate quam. Aliquam sollicitudin hendrerit erat at eleifend. Vivamus tincidunt felis vitae metus suscipit non volutpat neque tempus. Phasellus sagittis accumsan tellus, vitae pharetra ligula rhoncus vitae.
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<td><center><i>Photobacterium Phosphoreum</i>lumP</center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/5/5a/5._photobacterium_phosphoreum_lumazine_protein_%28lumP%29.zip">Download</a></center></td>
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<td><center>ptrp</center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/9/93/Ptrp.zip">Download</a></center></td>
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Here lorem ipsum dolor sit amet, consectetur adipiscing elit. Praesent nec nisi nec elit volutpat sollicitudin. Sed cursus venenatis egestas. In mollis vehicula dictum. Sed id lacus vitae tortor commodo pharetra porttitor ut purus. Nullam euismod urna at felis accumsan sit amet viverra leo fermentum. Phasellus ac molestie sapien. In in neque purus. Cras eget lorem arcu. Aliquam ornare tellus vel nisi commodo molestie. Aliquam aliquet magna nisl. Quisque velit nulla, adipiscing ut sagittis id, luctus viverra leo. Nam nunc nisl, euismod eu dignissim nec, eleifend ut augue. Phasellus tempor condimentum ipsum, ut molestie lorem suscipit vel. Nulla luctus turpis eget neque facilisis eget sodales ante tincidunt. Praesent congue lobortis mollis. Maecenas non augue id mi dignissim luctus. Donec quis volutpat lectus. Mauris condimentum sodales luctus. Cras eget lorem arcu. Aliquam ornare tellus vel nisi commodo molestie. Aliquam aliquet magna nisl. Quisque velit nulla, adipiscing ut sagittis id, luctus viverra leo. Donec quis volutpat lectus. Mauris condimentum sodales luctus. Cras eget lorem arcu. Aliquam ornare tellus vel nisi commodo molestie. Aliquam aliquet magna nisl. Quisque velit nulla, adipiscing ut sagittis id, luctus viverra leo.
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<td><center>nsrR and nir promoter from <i>N. Europaea</i></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/0/02/NsrR_and_nir_promoter_from_N._Europaea.zip">Download</a></center></td>
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<td><center><i>Xenorhabdus luminescens luciferase</i></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/2/29/Primer-_Xenorhabdus_luminescens_luciferase.zip">Download</a></center></td>
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<img src="http://www.scharfphoto.com/fine_art_prints/archives/199812-026-Staph-Bacteria.jpg" style="margin-left:10px;"width="370" height="300">
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<td><center><i>PETN reductase <i>(onr)</i></center></td>
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<td><center><a href="https://static.igem.org/mediawiki/2009/9/90/Primer_-_PETN_reductase_%28onr%29.zip">Download</a></center></td>
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<font size=2>Edinburgh University iGem Team 2009</font>
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<font size=2>Edinburgh University iGEM Team 2009</font>
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Latest revision as of 23:03, 21 October 2009

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Biology - Material and Methods
Protocol Name
Download Link
Making Competent Cells
Download
Transforming E. coli cells by heat shock
Download
Transforming E. coli cells by CaC12
Download
Transducing of E. coli cells
Download
Making P1 lysate
Download
Storing Cell Cultures
Download
PCR using Kod Polymerase
Download
Fusion PCR
Download
Sequencing protocol
Download
Building Composite BioBrick Parts
Download
Primers
Download Link
TNT receptors 1 and 3
Download
Trz fusion protein
Download
yeaR and ytfE upstream region
Download
Photobacterium Phosphoreum lux operon
Download
Photobacterium PhosphoreumlumP
Download
ptrp
Download
nsrR and nir promoter from N. Europaea
Download
Xenorhabdus luminescens luciferase
Download
PETN reductase (onr)
Download
Edinburgh University iGEM Team 2009

Retrieved from "http://2009.igem.org/Team:Edinburgh/biology(materialsandmethods)"