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Team:Newcastle/Labwork/13 August 2009
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No DNA was added to this plate, only <i>B.subtilis</i>. Therefore no colonies were expected to be seen. | No DNA was added to this plate, only <i>B.subtilis</i>. Therefore no colonies were expected to be seen. | ||
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[[Image:Newcastle 14 August BSub Transformation test h2o.jpg|thumb|250px|left|The transformation control (water) plate.]] | [[Image:Newcastle 14 August BSub Transformation test h2o.jpg|thumb|250px|left|The transformation control (water) plate.]] | ||
Revision as of 18:52, 10 October 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Formal Lab Session - 13th August 2009
Sporulation Tuning/Chassis Team
Summary
Yesterday's germination attempt seems to have worked, but results are not yet clear. This may have been due to a calculation error for the lysozyme, where instead of adding 40ul of stock lysozyme per ml of buffer solution, 4ul of stock lysozyme was added instead.
Therefore, on Monday, we intend to redo the experiment. See Monday, 17th August for more details.
Today we are trying to transform Bacillus subtilis with gfp-rrnb integration vector using the Transformation Protocol as well as following the changes which the Metal Sensor Team implemented. Metal sensor team kindly inoculated B. subtilis cells into flask tubes and placed them into the shaking incubator. The overnight cultures were labelled 1, 2 and 3, while the control was labelled as 4. Our team used overnight culture tube 3 and control tube 4.
Results
Following an illustration which clearly explains what to plate out from the Bacillus Transformation Protocol, we plated 4 plates, 3 of which contained the antibiotic chloramphenicol, and LB, and 1 containing just LB. The plate which contained just LB was the control plate.
The following pictures show the results of our team's transformations, which were a success.
No DNA was added to this plate, only B.subtilis. Therefore no colonies were expected to be seen.
Instead of adding DNA, water was added instead. Therefore, no colonies should be growing on this plate either.
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News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
