Project > OR Gate and Output
Design
The OR gate and output module is constructed as below:
Salicylate promter is placed upstream of the PO promoter, the double promoter construct can act as an OR Gate, either PhiR73 or salicylate can activate the promoter.
In Cambridge 2007 Amplifier project, the PhiR73 activator and the PO promoter is used to amplify signal from an inducible promoter.
In order to make the transcription level from PO promoter similar to that of the sal promoter, the activator is designed to be on a lower copy plasmid than the reporter. This is also discussed in the second stage assembly section.
Testing
In order to test whether the OR Gate works, we constructed the following 2 strains of E.coli to test each of the two promoters.
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