Team:TUDelft/16 September 2009

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(16 September 2009)
(Sriram)
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Today I checked in gel (see below) that the small colonies of LacI generator and Normal RBS control has the plasmids of correct sizes.
Today I checked in gel (see below) that the small colonies of LacI generator and Normal RBS control has the plasmids of correct sizes.
Hence with the correct LacI generator I ligated the control plasmids, Plasmid 1 of negative cascade and locks and keys.
Hence with the correct LacI generator I ligated the control plasmids, Plasmid 1 of negative cascade and locks and keys.
 +
Then they were electroporated to cells.
===Tim Weenink===
===Tim Weenink===

Revision as of 00:08, 22 October 2009

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16 September 2009

Sriram

Today I checked in gel (see below) that the small colonies of LacI generator and Normal RBS control has the plasmids of correct sizes. Hence with the correct LacI generator I ligated the control plasmids, Plasmid 1 of negative cascade and locks and keys. Then they were electroporated to cells.

Tim Weenink

Ligated the following constructs: !Y and !X and transformed the E. Coli K12 strain with them.