Lab Notebook

24 July 2009

Sriram

The cultures were grown in all the culture tubes [R0040, J23008, J23031, B0015, K081013, S03335, S03473, I714031, E0840, J23100]incubated yesterday except RBS[B0034] and pLacI[R0010], which may be because the colony we picked for those biobricks were not perfect. The plasmid DNA was extracted from all the grown cultures and stored in -20°C freezer.

The biobricks received from MIT for Delay [S03335, S03473] which were streaked yesterday have the colonies for future.

Tim Vos

Glycerol stocks were prepared for the remaining cultures grown in all the culture tubes [R0040, J23008, J23031, B0015, K081013, S03335, S03473, I714031, E0840, J23100]that were incubated yesterday and stored in -80°C freezer.

Daniel

Since the top10 competent cells newly prepared by us using the Openwetware protocol, didn't transform well for Tim Weenink we are in a bit crisis for competent cells. Anyway to make sure whether the cells work or not I did transformation of Biobricks RBS[B0034] and pLacI[R0010] in the newly created top10 competent cells and kept in drawer to grow in the weekend.

Orr

Today, I performed the mfold analysis to find out the secondary structures of the key3c and the lock3c. I also started looking at the COSMIC code that is intended to model conjugation. So far I only downloaded the source codes, and still need to find out how to run it.

Calin

Updated Matlab code to fix mRNA / protein confusion. Got growth in all tubes and plates. MIT parts placed in fridge. Tube cultures went to Sriram's miniprep for plasmid extraction. Did some GFP / RFP non-quantitative measurements. These can be done in the gel imaging machine.