Team:TUDelft/29 July 2009

From 2009.igem.org

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{| border="1" align="center"
{| border="1" align="center"
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| Well || Part || Expected Size
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| Well || Part || Expected Plasmid Size || Status
|- align="center"
|- align="center"
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| 1 || BBa_K142205 PCR ||  
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| 1 || BBa_K142205 PCR || ||  
|- align="center"
|- align="center"
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| 2 || BBa_K142202 PCR ||  
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| 2 || BBa_K142202 PCR ||  ||  
|- align="center"
|- align="center"
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| 3 || BBa_B0015 PCR ||  
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| 3 || BBa_B0015 PCR ||  ||  
|- align="center"
|- align="center"
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| 4 || BBa_R0040 PCR ||  
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| 4 || BBa_R0040 PCR ||  ||  
|- align="center"
|- align="center"
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| 5 || BBa_K145201 PCR ||  
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| 5 || BBa_K145201 PCR ||  ||  
|- align="center"
|- align="center"
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| 6 || *I3 uncut ||  
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| 6 || *I3 uncut ||  ||  
|- align="center"
|- align="center"
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| 7 || *I3 BglI cut ||  
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| 7 || *I3 BglI cut ||  ||  
|- align="center"
|- align="center"
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| 8 || *I3 BglI+I-SceI cut ||  
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| 8 || *I3 BglI+I-SceI cut ||  ||  
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|- align="center"
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| 9 || *I6 uncut ||  
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| 9 || *I6 uncut ||  ||  
|- align="center"
|- align="center"
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| 10 || *I6 BglI cut ||  
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| 10 || *I6 BglI cut ||  ||  
|- align="center"
|- align="center"
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| 11 || *I7 BglI+I-SceI cut ||  
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| 11 || *I7 BglI+I-SceI cut ||  ||  
|- align="center"
|- align="center"
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| 12 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  
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| 12 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  ||  
|- align="center"
|- align="center"
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| 13 || ||  
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| 13 || pSB1AK3 I || 3864 || <font color=red>&#10006;</font>
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| 14 || ||  
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| 14 || pSB1AK3 II || 3864 || <font color=red>&#10006;</font>
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| 15 ||  ||  
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| 15 || &lambda;p-GFP I ||  ||  
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| 16 ||  ||  
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| 16 || &lambda;p-GFP II ||  ||  
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| 17 ||  ||  
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| 17 || pLacI ||  ||  
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| 18 || ||  
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| 18 || rbs || 2091 || <font color=limegreen>&#10004;</font>
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|- align="center"
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| 19 || ||
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|- align="center"
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| 20 ||  ||
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|}
|}

Revision as of 18:33, 31 July 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

29 July 2009

Calin

Checked concentrations from yesterday's miniprep.

Part Concentration (ng/uL)
pSB1AK3 I 92
pSB1AK3 II 77
lambda_p GFP I 119
lambda_p GFP II 108
pLacI 56.7
RBS 95.2

Todays gel:

Gel29072009.jpg


Well Part Expected Plasmid Size Status
1 BBa_K142205 PCR
2 BBa_K142202 PCR
3 BBa_B0015 PCR
4 BBa_R0040 PCR
5 BBa_K145201 PCR
6 *I3 uncut
7 *I3 BglI cut
8 *I3 BglI+I-SceI cut
9 *I6 uncut
10 *I6 BglI cut
11 *I7 BglI+I-SceI cut
12 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
13 pSB1AK3 I 3864 ✖
14 pSB1AK3 II 3864 ✖
15 λp-GFP I
16 λp-GFP II
17 pLacI
18 rbs 2091 ✔

Orr

Got the source code in R for the modelling of conjugation, and already looked at how it works.

Retrieved from "http://2009.igem.org/Team:TUDelft/29_July_2009"