Team:TUDelft/29 July 2009

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Lab Notebook

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29 July 2009

Calin

Checked concentrations from yesterday's miniprep.

Part Concentration (ng/uL)
pSB1AK3 I 92
pSB1AK3 II 77
lambda_p GFP I 119
lambda_p GFP II 108
pLacI 56.7
RBS 95.2

Todays gel:

Gel29072009.jpg


Well Part Expected Size
1 BBa_K142205 PCR
2 BBa_K142202 PCR
3 BBa_B0015 PCR
4 BBa_R0040 PCR
5 BBa_K145201 PCR
6 *I3 uncut
7 *I3 BglI cut
8 *I3 BglI+I-SceI cut
9 *I6 uncut
10 *I6 BglI cut
11 *I7 BglI+I-SceI cut
12 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
13
14
15
16
17
18
19
20

Orr

Got the source code in R for the modelling of conjugation, and already looked at how it works.