Team:UNICAMP-Brazil/Notebooks/August 10

From 2009.igem.org

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(ColiGuard)
 
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{{:Team:UNICAMP-Brazil/inc calendar}}
{{:Team:UNICAMP-Brazil/inc calendar}}
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Today we ressuspended the chosen BioBricks according to the standard protocol. Each undergraduated student was responsible to ressuspend a set of BioBricks. All of them was transfomed into electrocompetent E. coli (DH10B)according to protocol X.
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__NOTOC__
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==''' ColiGuard '''==
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====Recovering the BioBricks====
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*<p style=”text-align:justify;”>Today we ressuspended the chosen BioBricks according to the standard protocol. Each undergraduated student was responsible to ressuspend a set of BioBricks. All of them were transfomed into electrocompetent ''E. coli'' cells (DH10B) according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3]. The cells were then plated in LB media containing the appropriate antibiotic.</p>
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'''Marcelo'''
'''Marcelo'''
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Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030  
Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030  
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'''Luige'''
'''Luige'''
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Part:BBa_J61107 - Ribosome Binding Site Family Member  
Part:BBa_J61107 - Ribosome Binding Site Family Member  
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'''Marcos'''
'''Marcos'''
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Part:BBa_B0014 - double terminator  
Part:BBa_B0014 - double terminator  
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'''Leonardo'''
'''Leonardo'''
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Part:BBa_J31003 - kanamycin resistance forward  
Part:BBa_J31003 - kanamycin resistance forward  
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Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)
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Part:BBa_E0040 - green fluorescent protein derived from jellyfish ''Aequeora victoria'' wild-type GFP (SwissProt: P42212)
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'''Victor'''
'''Victor'''
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Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata  
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Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from ''Discosoma striata''
Part:pSB1A3 -  High copy BioBrick assembly plasmid  
Part:pSB1A3 -  High copy BioBrick assembly plasmid  

Latest revision as of 00:29, 22 October 2009

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ColiGuard

Recovering the BioBricks

  • Today we ressuspended the chosen BioBricks according to the standard protocol. Each undergraduated student was responsible to ressuspend a set of BioBricks. All of them were transfomed into electrocompetent E. coli cells (DH10B) according to Protocol 3. The cells were then plated in LB media containing the appropriate antibiotic.


Marcelo

Part:BBa_B0030 - RBS.1 (strong) -- modified from R. Weiss

Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030


Luige

Part:BBa_J61100 - Ribosome Binding Site Family Member

Part:BBa_J61107 - Ribosome Binding Site Family Member


Marcos

Part:BBa_B0010 - T1 from E. coli rrnB

Part:BBa_B0014 - double terminator


Leonardo

Part:BBa_B0015 - double terminator (B0010-B0012)

Part:BBa_R0040 - TetR repressible promoter

Part:BBa_J23100 - constitutive promoter family member

Part:BBa_J23107 - constitutive promoter family member


Fabiana

Part:BBa_J23109 - constitutive promoter family member

Part:BBa_J31007 - tetracycline resistance protein TetA(C)

Part:BBa_J31003 - kanamycin resistance forward

Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)


Victor

Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata

Part:pSB1A3 - High copy BioBrick assembly plasmid

Part:pSB1AK3 - High copy BioBrick assembly plasmid

Part:pSB3K3 - Low to medium copy BioBrick standard vector

Part:pSB6A1 - pBR322 converted to BioBrick vector (formally BBa_I739201)