EPF-Lausanne/27 July 2009
From 2009.igem.org
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Wet Lab
LOVTAP gene have been amplified again and runned on a gel to be sure to have the correct insert.
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)] was digested, runned on a gel (which displayed the correct band) and extracted with the gel extraction kit, this step didn't worked well.
LOVTAP gene have been than digested, purified and runned on a gel.
We already had a digested [http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)].
Finally we ligated overnight at 4°C the digested LOVTAP in both [http://partsregistry.org/Part:BBa_B0015 terminators], the one digested-extracted today and the one already digested-extracted.
People in the lab
- Gab, Christian, Mélanie, Nicolas, Nath
![](https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png)
![](https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png)