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Team:Kyoto/GSDD/Notebook/0727-0802
From 2009.igem.org
- 0727-0802
- Construction.
- 0803-0809
- Construction.
- 0810-0816
- Construction.
- 0817-0823
- Construction.PCR.
- 0824-0830
- Construction.PCR.
- 0831-0906
- Construction.PCR.MPR.
- 0907-0913
- Construction.PCR.MPR.
- 0914-0920
- Construction.PCR.MPR.
- 0921-0927
- Construction.PCR.MPR.
- 0928-1004
- Construction.PCR.MPR.
- 1005-1011
- Construction.PCR.MPR.
- 1012-1018
- Construction.MPR.Observation.
0727-0802 : Construction.
TuesDay, 28 July - Wednesday, 29 July
To Do
- Transformation
- 3-M-9
- 3-I-9
- 1-A-4
- 1-O-2
- 3-O-22(retry)
- 3-M-2(retry)
- 3-O-17(retry)
Results
- Colony
- 3-M-9
- None
- 3-I-9
- Observed
- 1-A-4
- Observed
- 1-O-2
- Observed
- 3-O-22(retry)
- None
- 3-M-2(retry)
- Observed
- 3-O-17(retry)
- None
Thursday, 30 July
To Do
- Transformation
- 3-O-17(retry)
- 3-M-9(retry)
Results
- Colony
- 3-O-17(retry)
- None
- 3-M-9(retry)
- Observed
Thursday, 30 July
To Do
- Miniprep Kit
- Restriction Enzyme Digestion (EcoR1, EcoR1 + Spe1)
- Electrophoresis
- 1-a-4
- 1-0-2
- 3-m-2
- 3-m-9
- 3-i-9
Results
Miniprep conc.
| NO. | Sample name | conc./(ng/ul) |
|---|---|---|
| 1 | 1-a-4 | 64.1 |
| 2 | 1-o-2 | 73.6 |
| 3 | 3-m-2 | 96.5 |
| 4 | 3-m-9 | 58.6 |
| 5 | 3-i-9 | 59.0 |
Electrophoresis
- No bands were observed.
| NO. | Sample name | parts length/bp | plasmid length/bp |
|---|---|---|---|
| 1 | 1-a-4 | 660 | 2079 |
| 2 | 1-o-2 | 1128 | 2079 |
| 3 | 3-m-2 | 253 | 2077 |
| 4 | 3-m-9 | 228 | 2079 |
| 5 | 3-i-9 | 438 | 2079 |
