Team:Kyoto/GSDD/Notebook/1012-1018

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0727-0802
Construction.
0803-0809
Construction.
0810-0816
Construction.
0817-0823
Construction.PCR.
0824-0830
Construction.PCR.
0831-0906
Construction.PCR.MPR.
0907-0913
Construction.PCR.MPR.
0914-0920
Construction.PCR.MPR.
0921-0927
Construction.PCR.MPR.
0928-1004
Construction.PCR.MPR.
1005-1011
Construction.PCR.MPR.
1012-1018
Construction.MPR.Observation.

1012-1018 : Construction.MPR.Observation.

Wednesday, 14 October

To Do

  1. Miniprep Kit
  2. Transformation yeast
  • (Byp4731, a GFP vector)

Results

Miniprep kit
sampleConc. /(ng/ul)
2M Lv2-4(5)134.8
4(4)-2M Lv2383.2
4(3)211.1
4(4)125.4
3(2)21101.7
3(2)2262.3
Transformation

After transformation, Colony were observed.

Saturday, 17 October

To Do

  1. Restriction enzyme digestion 1-10
  • Electorophoresis
  • Restriction enzyme digestion
  • Eloectorophoresis

Results

Restriction enzyme digestion
  • 1-10(E-P)

Kyoto 1017 1.png

  • 1,2(E-S)

Kyoto 1017 2.png

Timer vector was constructed completely.


Sunday, 18 October

To Do

  1. Transformation yeast timer vector
  2. Observation of yeast by microscope
  • By782
  • By782 transformed byp4731, a GFP vector

Results

Microscope
  • By782 transformed byp4731
  • DIC

Kyoto 1018 1.png

  • By782 transformed byp4731
  • 288nm

Kyoto 1018 2.png

  • By782
  • DIC

Kyoto 1018 3.png

  • By782
  • 488nm

Kyoto 1018 4.png


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