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Team:Kyoto/GSDD/Notebook/0824-0830
From 2009.igem.org
- 0727-0802
- Construction.
- 0803-0809
- Construction.
- 0810-0816
- Construction.
- 0817-0823
- Construction.PCR.
- 0824-0830
- Construction.PCR.
- 0831-0906
- Construction.PCR.MPR.
- 0907-0913
- Construction.PCR.MPR.
- 0914-0920
- Construction.PCR.MPR.
- 0921-0927
- Construction.PCR.MPR.
- 0928-1004
- Construction.PCR.MPR.
- 1005-1011
- Construction.PCR.MPR.
- 1012-1018
- Construction.MPR.Observation.
0824-0830 : Construction.PCR.
Tuesday, 25 August
To Do
- PCR
- Electrophoresis
- Gel Extraction kit
- 3-O-22
- 1-O-2
Results
PCR
| templateDNA sample | conc./(ng/ul) |
|---|---|
| 3-O-22 | 79.1 |
| 1-O-2 | 75.5 |
- 3-O-22 PCR product (1,2)
- 1232bp
- 1-O-2 PCR product (3,4)
- 462bp
Extraction kit
| sample | conc./(ng/ul) |
|---|---|
| (1)1 | 84.3 |
| (1)2 | 88.9 |
| (4)1 | 181.3 |
| (4)2 | 126.2 |
Wednesday, 26 August
To Do
- Restriction Enzyme Digestion
- purification kit
- ligation
- Transformation
- 3-O-22
- 1-O-2
Results
PCR
| templateDNA sample | conc./(ng/ul) |
|---|---|
| (1)2 | 22.3 |
| (4)2 | 58.7 |
transformation
- colony
- p-2(1)2
- none
- p-2(4)2
- observed
We are going to try transformation of p【2】(4)② again.
Thursday, 27 August
To Do
- (use p【2】(1)② after 0826 ligation)
- Transformation
- (use 【2】(1)① after 0825PCR, Gel extraction)
- Restriction Enzyme Digestion
- PCR purification kit
- Ligation
- Transformation
Results
Gel extraction kit
| templateDNA sample | conc./(ng/ul) |
|---|---|
| 1-(1)A | 38.1 |
| 1-(4)B | 21.4 |
transformation
- colony
- p-2(1)2
- observed
- p-2(1)1A
- none
- p-2(1)1B
- observed
We are going to make master plates of these sample.
Thursday, 27 August
To Do
- 2(4)1(0825PCR)
- Restriction Enzyme Digestion
- PCR purification kit
- ligation
- transformation
- p-2(1)1
- p-2(1)2
- p-2(4)2
(0825PCRed)
- Make master plates.
Results
PCR purificaiton kit
| sample | conc./(ng/ul) |
|---|---|
| (4)1 | 33.7 |
transformation
No colony was observed.
We are going to try transformation of p【2】(4)① again.
