Team:Newcastle/IntroductoryLabwork/27 July 2009

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Introductory Lab Session - 27th July 2009

Introduction

Last week the team had completed the ethanol precipitation of the GFP and RFP (in plasmids pSB1A2 and pSB1AT3 respectively) DNA samples. They are midi-prepped plasmid DNA samples extracted using Sigma-Aldrich's GenElute Midi-prep kit, however the ethanol present in Wash Solution 2 hadn't been completely removed so ethanol precipitation was necessary. With the ethanol removed, it should be analyse the samples through DNA gel electrophoresis.

Practical Outline

1. Run the GFP and RFP plasmid DNA samples on gel through DNA gel electrophoresis and analyse.

Analysing the DNA samples through DNA Gel Electrophoresis

June M T W T F S S [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/1_June_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/7_June_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/14_June_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/21_June_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/22_June_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/23_June_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/28_June_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/29_June_2009&action=edit 29] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/30_June_2009 30]

July M T W T F S S     [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/1_July_2009 1] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/2_July_2009 2] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/5_July_2009&action=edit 5] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/6_July_2009 6] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/7_July_2009 7] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/8_July_2009 8] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/9_July_2009 9] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/12_July_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/13_July_2009&action=edit 13] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/14_July_2009 14] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/15_July_2009 15] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/16_July_2009 16] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/17_July_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/19_July_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/20_July_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/21_July_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/22_July_2009&action=edit 22] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/23_July_2009 23] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/24_July_2009 24] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/25_July_2009 25] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/26_July_2009&action=edit 26] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/27_July_2009 27] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/31_July_2009&action=edit 31]