Team:TUDelft/Modeling Conjugation Plots

From 2009.igem.org

Conjugation Modeling Plots

Here is a selection of the various simulations made.

SIM1

Parameters used:

g_r = 20 μm/hr

λ = 5.76 CFU/mm² (N_d = 10000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.	Donors at T = 0 hr.
Transconjugants at T = 0.1 hr.	Transconjugants at T = 0.2 hr.
Transconjugants at T = 0.3 hr.	Transconjugants at T = 0.4 hr.
Transconjugants at T = 0.5 hr.	Transconjugants at T = 0.6 hr.
Transconjugants at T = 0.7 hr.	Transconjugants at T = 0.8 hr.
Transconjugants at T = 0.9 hr.	Transconjugants at T = 1.0 hr.

SIM2

Parameters used:

g_r = 30 μm/hr

λ = 5.76 CFU/mm² (N_d = 10000)

Ratio of Donors to Recipients = 1

Animation of conjugation showing all colonies:

Recipients at T = 0 hr.	Donors at T = 0 hr.
Transconjugants at T = 0.1 hr.	Transconjugants at T = 0.2 hr.
Transconjugants at T = 0.3 hr.	Transconjugants at T = 0.4 hr.
Transconjugants at T = 0.5 hr.	Transconjugants at T = 0.6 hr.
Transconjugants at T = 0.7 hr.	Transconjugants at T = 0.8 hr.
Transconjugants at T = 0.9 hr.	Transconjugants at T = 1.0 hr.

SIM3

Parameters used:

g_r = 40 μm/hr

λ = 5.76 CFU/mm² (N_d = 10000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.	Donors at T = 0 hr.
Transconjugants at T = 0.1 hr.	Transconjugants at T = 0.2 hr.
Transconjugants at T = 0.3 hr.	Transconjugants at T = 0.4 hr.
Transconjugants at T = 0.5 hr.	Transconjugants at T = 0.6 hr.
Transconjugants at T = 0.7 hr.	Transconjugants at T = 0.8 hr.
Transconjugants at T = 0.9 hr.	Transconjugants at T = 1.0 hr.

SIM4

Parameters used:

g_r = 50 μm/hr

λ = 5.76 CFU/mm² (N_d = 10000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.	Donors at T = 0 hr.
Transconjugants at T = 0.1 hr.	Transconjugants at T = 0.2 hr.
Transconjugants at T = 0.3 hr.	Transconjugants at T = 0.4 hr.
Transconjugants at T = 0.5 hr.	Transconjugants at T = 0.6 hr.
Transconjugants at T = 0.7 hr.	Transconjugants at T = 0.8 hr.
Transconjugants at T = 0.9 hr.	Transconjugants at T = 1.0 hr.

SIM5

Parameters used:

g_r = 60 μm/hr

λ = 5.76 CFU/mm² (N_d = 10000)

Ratio of Donors to Recipients = 1

Animation of conjugation showing all colonies:

Recipients at T = 0 hr.	Donors at T = 0 hr.
Transconjugants at T = 0.1 hr.	Transconjugants at T = 0.2 hr.
Transconjugants at T = 0.3 hr.	Transconjugants at T = 0.4 hr.
Transconjugants at T = 0.5 hr.	Transconjugants at T = 0.6 hr.
Transconjugants at T = 0.7 hr.	Transconjugants at T = 0.8 hr.
Transconjugants at T = 0.9 hr.	Transconjugants at T = 1.0 hr.

SIM6

Parameters used:

g_r = 30 μm/hr

λ = 1.44 CFU/mm² (N_d = 1000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.	Donors at T = 0 hr.
Transconjugants at T = 0.1 hr.	Transconjugants at T = 0.2 hr.
Transconjugants at T = 0.3 hr.	Transconjugants at T = 0.4 hr.
Transconjugants at T = 0.5 hr.	Transconjugants at T = 0.6 hr.
Transconjugants at T = 0.7 hr.	Transconjugants at T = 0.8 hr.
Transconjugants at T = 0.9 hr.	Transconjugants at T = 1.0 hr.

SIM7

Parameters used:

g_r = 30 μm/hr

λ = 2.88 CFU/mm² (N_d = 5000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.	Donors at T = 0 hr.
Transconjugants at T = 0.1 hr.	Transconjugants at T = 0.2 hr.
Transconjugants at T = 0.3 hr.	Transconjugants at T = 0.4 hr.
Transconjugants at T = 0.5 hr.	Transconjugants at T = 0.6 hr.
Transconjugants at T = 0.7 hr.	Transconjugants at T = 0.8 hr.
Transconjugants at T = 0.9 hr.	Transconjugants at T = 1.0 hr.

SIM8

Parameters used:

g_r = 20 μm/hr

λ = 11.53 CFU/mm² (N_d = 20000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.	Donors at T = 0 hr.
Transconjugants at T = 0.1 hr.	Transconjugants at T = 0.2 hr.
Transconjugants at T = 0.3 hr.	Transconjugants at T = 0.4 hr.
Transconjugants at T = 0.5 hr.	Transconjugants at T = 0.6 hr.
Transconjugants at T = 0.7 hr.	Transconjugants at T = 0.8 hr.
Transconjugants at T = 0.9 hr.	Transconjugants at T = 1.0 hr.

SIM9

Parameters used: g_r = 20 μm/hr

λ = 23.06 CFU/mm² (N_d = 40000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.	Donors at T = 0 hr.
Transconjugants at T = 0.1 hr.	Transconjugants at T = 0.2 hr.
Transconjugants at T = 0.3 hr.	Transconjugants at T = 0.4 hr.
Transconjugants at T = 0.5 hr.	Transconjugants at T = 0.6 hr.
Transconjugants at T = 0.7 hr.	Transconjugants at T = 0.8 hr.
Transconjugants at T = 0.9 hr.	Transconjugants at T = 1.0 hr.

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