Team:UNICAMP-Brazil/Notebooks/August 31

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YeastGuard

Gallus gallus RNA

Quantification

  • We have verified the concentration of RNA we got in the RNA extractions from the G. gallus organs. The following table shows the results.

Samples ng/uL 260/280 260/230
Blood 1 743.42 2.01 1.78
Blood 2 604.84 1.99 1.54
Blood 3 1814.10 1.97 1.88
Spleen 1 3607.90 1.96 1.57
Spleen 2 3715.95 1.94 1.55
Spleen 3 4213.93 1.78 1.69
Spleen 4 4243.37 1.76 1.69
Oviduct 1 4362.04 1.66 1.41
Oviduct 2 3498.69 1.87 1.16
Oviduct 3 3601.43 1.84 1.13
Oviduct 4 3568.66 1.84 1.12

  • The concentration values are ok, but the ratios 260/280 and 260/230 are not that good. Let’s find out what we see in the agarose gel.

Raíssa

Agarose gel

  • We have chosen the best samples from each organ to verify the quality in the gel. The samples are: Blood1, Blood3, Spleen1 and Oviduct2. We had problems with our UV transilluminator and didn’t manage to take pictures of the gel. =[

  • But we are going to tell you what we found! The bands were there, especially in the spleen and oviduct samples, not perfect, but we bet they are ok for cDNA synthesis.

Taís

Terminator biobrick and RBS+CFP ligation

  • We verified using electrophoresis, the digestion products of both parts digested yesterday (Terminator and RBS+CFP).

  • The gel showed we had no success with the digestions. Two inespecific bands appeared.

‎

Wesley




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