Team:UNICAMP-Brazil/Notebooks/July 25

From 2009.igem.org

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MicroGuards

Primer design

These are the primers that we are going to use in our project. How long will it take until they´re sent to us?? :)


Col-R CTACTAGTACCAGAAATTCAGCCTCGGTA

Anticol-F TTCTAGAGGTGGTAGGGAACGCTTTGAA

Anticol-R CTACTAGTAACCGGCATTCATATTTCAGC

HLY-F TTCTAGAGATAAAGATGGTCGAGA

HLY-R CTACTAGTCTAACGTTCACGTAAACTT

LYS-F TTCTAGAATGGTTGAAATCAATAATC

LYS-R CTACTAGTTACATCAATCTCTCTGACC

FinOF-XbaI TTCTAGAGATGACAGAGCAGAAACGACCGG

FinOR-SpeI CTACTAGTATTATTTCTCATCAAGCACGGCC

FinPF-XbaI TTCTAGAGTGACGAGCATGTTTTTGTTGAA

FinPR-SpeI CTACTAGTAAAAATCGCCGATGCAGGGAGAC

Ppy-F-1 TTCTAGAGACGAGAAGGCTATGTGTATCA

Ppy-F-2 TTCTAGAGTTTCCGTCTATTTACCTTTTCTG

Ppy-R CTACTAGTATAACACCTCCCGCTGTTTAT

KlJEN1-F TTCTAGATCAACCATGAATAACAACAAT

KlJEN1-R CTACTAGTATAATGCTATGGTGCGTGT

Prom-KlJEN1-F TTCTAGATGATTGGCAAACCTGTTGT

Prom-KlJEN1-R CTACTAGTGGTTGAAATCTGTTTTGGTTTT

Prom-KlDLD-F TTCTAGAGGTCGCTCTTTCTCTCTCTCAT

Prom-KlDLD-R CTACTAGTTGAGTTGCGTTTCTGGTCTTT

GgLYSO_F TTCTAGAATGAGGTCTTTGCTAATCTTGG

GgLYSO_R CTACTAGTTTTTAATGAGGGATGCGTTT

Cre-F TCCAATTTACTGACCGTACACC

FSTK_UP_VF2 CACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTTGCCACCTGACGTCTAAGAA

FSTK_UP_VR TACAGGCTTTTCCTGCCCGATGCTAAAACGGACAACAGTACCATTACCGCCTTTGAGTGAGC

FSTK_DOWN_VF2 TCGTATGCCGGATAAGGCGCGGTAGCGTCGCATCCGGCACTCTGCCACCTGACGTCTAAGAA

FSTK_DOWN_VR AAATTACGTCACTCCCGATCGGGAGTTCCCTCTACTCTCTATATTACCGCCTTTGAGTGAGC

Slippage_Cre-R TGGTGTACGGTCAGTAAATTGGAGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTCAT