The digestion with the HindIII restriction enzyme was performed according to Protocol 14. In place of the second enzyme that is used in this reaction, it was used the same volume of autoclaved distilled water.
Gabriel
PY Promoter - Transformation
After the O/N period we transformed the ligations PY1 + BBa_J23100 and PY2 + BBa_J23100 into electrocompetent E. coli bacteria, strain DH10B. We followed Protocol 3 (Electroporation) without modifications.
After the transformation we plated the transformed cells in LB-AMP plates and let them grow at 37ºC for an O/N period.
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