Team:UNICAMP-Brazil/Notebooks/September 24

From 2009.igem.org

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

ColiGuard

E. coli transformation with F plasmid

  • E. coli DH10B electrocompetent cells were transformed using the recircularized F plasmid, according to Protocol 3.

  • After the incubation time, 100 μl of medium containing the transformed cells were plated in LB-AMP-Xgal culture plates, and incubated O/N at 37°C.

Gabriel

finO and finP - Still Trying to Confirm our Biobricks

  • After the miniprep procedure, we performed PCRs with finO and finP's designed primer, in order to confirm that our inserted fragment is indeed contained in the vector.

  • As we had already got an amplified fragment of expected size from those colonies, we expect that those PCRs results in a successful amplification of our fragments. Moreover, as we aimed in reducing the amount of genomic DNA, we should got less inespecific amplifications.


Marcelo


CeaB and CeiB: Miniprep and transformation

  • Today, we performed miniprep (Protocol 2) of the transformed cells and PCR again, so that avoids unspecific amplification. We didn’t get the expected band size again.


Luige