Team:UQ-Australia/Notebook/Colony pick procedure
From 2009.igem.org
Colony Picking Procedure
Micro-culture
3ml of LB Broth 3ml of Antibiotic (depends on the antibiotic resistance gene on the plasmid)
1. Touch a single colony with a pipette tip and place the entire tip into the tube
2. Repeat 5 times or for as many colonies which are present
3. Place in incubator/shaker overnight at 37'C