Team:Paris/Transduction testing Trans

From 2009.igem.org

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the transduction system of the fec operon (FecA, FecR, FecI).
the transduction system of the fec operon (FecA, FecR, FecI).
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[[Image:Paris_construc_6_pfec.jpg  |700px|thumb|left|plasmid = ]]
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[[Image:Paris_construc_2_feca.jpg  |700px|thumb|left|plasmid = ]]
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Time required : A lOT !!!!!
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Experiments ran :
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{|
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|- style="background: #0a3585; text-align: center; color:white; font-weight:bold; "
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|width= 300px| row 1
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|width=300px| row 2
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|width=300px| row 3
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|- style="background: #bebebe; text-align: center;"
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|'''PCR :'''
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|'''PCR :'''
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|'''PCR :'''
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|- style="background: #d8d8d8; text-align: center;"
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|'''Verification on gel :'''
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ok
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|'''Verification on gel :'''
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ok
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|'''Verification on gel :'''
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ok
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|- style="background: #bebebe; text-align: center;" 
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|'''Purification on gel :'''
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ok
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|'''Purification on gel :'''
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ok
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|'''Purification on gel :'''
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ok
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|- style="background: #d8d8d8; text-align: center;"
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|'''digestion:'''
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|'''digestion:'''
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|'''digestion:'''
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|- style="background: #bebebe; text-align: center;" 
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|'''verification digestion:'''
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ok
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|'''verification digestion:'''
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ok
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|'''verification digestion:'''
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ok
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|- style="background: #d8d8d8; text-align: center;"
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|'''ligation:'''
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|'''ligation:'''
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|'''ligation:'''
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|- style="background: #bebebe; text-align: center;" 
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|'''PCR colo :'''
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ok
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|'''PCR colo :'''
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ok
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|'''PCR colo :'''
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ok
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|- style="background: #d8d8d8; text-align: center;"
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|'''miniprep:'''
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clone :
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|'''miniprep:'''
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clone :
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|'''miniprep:'''
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clone :
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|- style="background: #bebebe; text-align: center;" 
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|'''sequencing :'''
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ok
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|'''sequencing :'''
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ok
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|'''sequencing :'''
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ok
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|}

Latest revision as of 11:47, 17 October 2009

iGEM > Paris > Reception > Testing > Transduction

WetLab

Main - Addressing - Production - reception -
Reception


Transduction

Transduction : the construction of the system that will be able de decypher the message.


the transduction system of the fec operon (FecA, FecR, FecI).


plasmid =


plasmid =



Time required : A lOT !!!!!



Experiments ran :

row 1 row 2 row 3
PCR :



PCR : PCR :


Verification on gel :

ok

Verification on gel :

ok

Verification on gel :

ok

Purification on gel :

ok

Purification on gel :

ok

Purification on gel :

ok


digestion:



digestion: digestion:
verification digestion:

ok

verification digestion:

ok

verification digestion:

ok


ligation:


ligation: ligation:


PCR colo :

ok

PCR colo :

ok

PCR colo :

ok


miniprep:

clone :

miniprep:

clone :

miniprep:

clone :


sequencing :

ok

sequencing :

ok

sequencing :

ok

Retrieved from "http://2009.igem.org/Team:Paris/Transduction_testing_Trans"