Team:Paris/Production testing tr2

From 2009.igem.org

(Difference between revisions)
(New page: <span/ id="bottom">[https://2009.igem.org/ iGEM ] > Paris > Production > Testing > [...)
(Destabilization)
 
(2 intermediate revisions not shown)
Line 15: Line 15:
== Destabilization ==
== Destabilization ==
-
part1 :
+
[[Image:Paris_construc_4_tr2.jpg |700px|center|plasmid = PSB3T5]]
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
Time required : A lOT !!!!!
 +
 
 +
 
 +
Experiments ran :
 +
 
 +
{|
 +
|- style="background: #0a3585; text-align: center; color:white; font-weight:bold; "
 +
|width= 300px| row 1
 +
|width=300px| row 2
 +
|width=300px| row 3
 +
 
 +
|- style="background: #bebebe; text-align: center;"
 +
|'''PCR :'''
 +
 
 +
 
 +
 
 +
 
 +
|'''PCR :'''
 +
|'''PCR :'''
 +
 
 +
 
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''Verification on gel :'''
 +
 
 +
ok
 +
 
 +
|'''Verification on gel :'''
 +
 
 +
ok
 +
 
 +
|'''Verification on gel :'''
 +
 
 +
ok
 +
 
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''Purification on gel :'''
 +
 
 +
ok
 +
 
 +
|'''Purification on gel :'''
 +
 
 +
ok
 +
 
 +
|'''Purification on gel :'''
 +
 
 +
ok
 +
 
 +
 
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''digestion:'''
 +
 
 +
 
 +
 
 +
 
 +
 
 +
|'''digestion:'''
 +
|'''digestion:'''
 +
 
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''verification digestion:'''
 +
 
 +
ok
 +
 
 +
|'''verification digestion:'''
 +
 
 +
ok
 +
 
 +
|'''verification digestion:'''
 +
 
 +
ok
 +
 
 +
 
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''ligation:'''
 +
 
 +
 
 +
 
 +
|'''ligation:'''
 +
|'''ligation:'''
 +
 
 +
 
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''PCR colo :'''
 +
 
 +
ok
 +
 
 +
|'''PCR colo :'''
 +
 
 +
ok
 +
 
 +
|'''PCR colo :'''
 +
 
 +
ok
 +
 
 +
 
 +
|- style="background: #d8d8d8; text-align: center;"
 +
|'''miniprep:'''
 +
 
 +
clone :
 +
 
 +
|'''miniprep:'''
 +
 
 +
clone :
 +
 
 +
|'''miniprep:'''
 +
 
 +
clone :
 +
 
 +
 
 +
|- style="background: #bebebe; text-align: center;" 
 +
|'''sequencing :'''
 +
 
 +
ok
 +
 
 +
|'''sequencing :'''
 +
 
 +
ok
 +
 
 +
|'''sequencing :'''
 +
 
 +
ok
 +
 
 +
|}

Latest revision as of 13:32, 17 October 2009

iGEM > Paris > Production > Testing > Destabilization

WetLab

Main - Addressing - Production - reception -
Production

Destabilization

plasmid = PSB3T5






Time required : A lOT !!!!!


Experiments ran :

row 1 row 2 row 3
PCR :



PCR : PCR :


Verification on gel :

ok

Verification on gel :

ok

Verification on gel :

ok

Purification on gel :

ok

Purification on gel :

ok

Purification on gel :

ok


digestion:



digestion: digestion:
verification digestion:

ok

verification digestion:

ok

verification digestion:

ok


ligation:


ligation: ligation:


PCR colo :

ok

PCR colo :

ok

PCR colo :

ok


miniprep:

clone :

miniprep:

clone :

miniprep:

clone :


sequencing :

ok

sequencing :

ok

sequencing :

ok