Team:UNICAMP-Brazil/Notebooks/September 13
From 2009.igem.org
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*<p style=”text-align:justify;”>After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent ''E. coli'' bacteria, strain DH10B. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3], without modifications.</p> | *<p style=”text-align:justify;”>After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent ''E. coli'' bacteria, strain DH10B. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3], without modifications.</p> | ||
*<p style=”text-align:justify;”>We then plated the transfomed cells in LB-AMP medium, and let them grow at 37ºC for an O/N period.</p> | *<p style=”text-align:justify;”>We then plated the transfomed cells in LB-AMP medium, and let them grow at 37ºC for an O/N period.</p> | ||
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+ | ''Marcelo'' | ||
====finP+pSB1A3==== | ====finP+pSB1A3==== |
Revision as of 15:34, 18 October 2009
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