Team:Paris/Protocols
From 2009.igem.org
(Difference between revisions)
Christophe.R (Talk | contribs) (→Protocols) |
Christophe.R (Talk | contribs) (→2. Adapted Protocols) |
||
(38 intermediate revisions not shown) | |||
Line 3: | Line 3: | ||
{{Template:Paris2009_menu}} | {{Template:Paris2009_menu}} | ||
- | |||
- | |||
- | + | ==Main== | |
- | + | ||
- | + | ||
<html> | <html> | ||
<style type="text/css"> | <style type="text/css"> | ||
#left-side { | #left-side { | ||
position: absolute; | position: absolute; | ||
- | top: | + | height: 23px; |
- | left: - | + | width: 30px; |
+ | top: 0px; | ||
+ | left: 40px; | ||
+ | margin-top:10px; | ||
+ | padding-top: 7px; | ||
+ | background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png); | ||
+ | z-index:4; | ||
} | } | ||
#middle-side { | #middle-side { | ||
- | height: | + | height: 25px; |
- | width: | + | width: 560px; |
position: absolute; | position: absolute; | ||
top: 0px; | top: 0px; | ||
- | left: | + | left: 60px; |
+ | margin-top:10px; | ||
+ | padding-top: 5px; | ||
background: #dadada; | background: #dadada; | ||
+ | z-index:5; | ||
} | } | ||
#right-side { | #right-side { | ||
position: absolute; | position: absolute; | ||
- | top: | + | height: 23px; |
- | left: - | + | width: 30px; |
+ | margin-top:10px; | ||
+ | padding-top: 7px; | ||
+ | top: 0px; | ||
+ | left: 600px; | ||
+ | background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png); | ||
+ | z-index:4; | ||
} | } | ||
a.menu_sub { | a.menu_sub { | ||
- | padding: | + | padding-left: 7px; |
+ | padding-right: 7px; | ||
+ | } | ||
+ | a.menu_sub_active { | ||
+ | padding-left: 7px; | ||
+ | padding-right: 7px; | ||
+ | color:#b0310e; | ||
+ | font-weight:bold; | ||
} | } | ||
</style> | </style> | ||
- | <div id="middle-side"><a class="menu_sub" href="https://2009.igem.org/Team:Paris/ProtocolsA# | + | <div id="left-side"></div> |
+ | <div id="middle-side"><center> | ||
+ | <a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Protocols#bottom"> Main </a>| | ||
+ | <a class="menu_sub" href="https://2009.igem.org/Team:Paris/ProtocolsA#bottom"> Microscope Protocols</a>| | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsB#bottom"> Adapted Protocols</a>| | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Protocols_Culture#bottom"> Culture Protocols</a>| | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsMB#bottom"> Molecular biology</a> | ||
+ | </center> | ||
</div> | </div> | ||
- | <div id=" | + | <div id="right-side"></div> |
- | </div> | + | |
- | + | ||
</html> | </html> | ||
- | |||
===1. Microscopy=== | ===1. Microscopy=== | ||
* [[Team:Paris/ProtocolsA#Protocols suggested | Protocols suggested]] | * [[Team:Paris/ProtocolsA#Protocols suggested | Protocols suggested]] | ||
Line 55: | Line 78: | ||
* [[Team:Paris/ProtocolsB#Adaptation of PureYield™ Plasmid Miniprep System (Promega) |Mini prep ]] | * [[Team:Paris/ProtocolsB#Adaptation of PureYield™ Plasmid Miniprep System (Promega) |Mini prep ]] | ||
* Gel/PCR Purification | * Gel/PCR Purification | ||
- | |||
- | |||
===3. Culture protocols=== | ===3. Culture protocols=== |
Latest revision as of 16:35, 20 October 2009
iGEM > Paris > Protocols > Main
Contents |
Main
1. Microscopy
- Protocols suggested
- [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]
2. Adapted Protocols
- DNA extraction
- Mini prep
- Gel/PCR Purification
3. Culture protocols
4. Molecular biology
- PCR
- PCR colony
- Gels
- Digestion
- Ligation