Team:Wisconsin-Madison/Notebook Protocols

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Latest revision as of 18:26, 20 October 2009

PROTOCOLS Plasmid Preparation by Alkaline Lysis Plasmid Preparation by Boiling Lysis Restriction Enzyme Mapping Transformation of Plasmids into Competent Cells Preparation of Competent Cells Overview of Cloning Protocol GREAT for New Teams! Polymerase Chain Reaction (PCR) Ligation Digestion - Cloning Loading Gel Protocol Sequencing Reaction Colony Polymerase Chain Reaction (PCR) Genomic DNA Extraction Safety

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 {{Wisconsin-Madison Template}}
 {{Wisconsin-Madison Note Template}}
+<br>
+{| class="wikitable" border="0" align="center" width=790px
+|style="text-align:left" style="vertical-|
+<center>
+ '''PROTOCOLS'''
+</center>
-=='''Protocols'''==
+* [[Team:Wisconsin-Madison/Plasmid Preparation by Alkaline Lysis|Plasmid Preparation by Alkaline Lysis]]
+* [[Team:Wisconsin-Madison/Plasmid Preparation by Boiling Lysis|Plasmid Preparation by Boiling Lysis]]
-<br>
+* [[Team:Wisconsin-Madison/Restriction Digestion|Restriction Enzyme Mapping]]
-Plasmid Preparation by Alkaline Lysis
+* [[Team:Wisconsin-Madison/Transformation of Plasmids into Competent Cells|Transformation of Plasmids into Competent Cells]]
-Plasmid Preparation by Boiling Lysis
+* [[Team:Wisconsin-Madison/Preparation of Competent Cells|Preparation of Competent Cells]]
-Transformation of Plasmids into Competant Cells
+* [[Team:Wisconsin-Madison/Cloning Protocols|Overview of Cloning Protocol]] ** GREAT for New Teams! **
-Preparation of Competent Cells
+* [[Team:Wisconsin-Madison/Polymerase Chain Reaction (PCR)|Polymerase Chain Reaction (PCR)]]
-Cloning Protocols
+* [[Team:Wisconsin-Madison/Ligation|Ligation]]
-Polymerase Chain Reaction (PCR)
+* [[Team:Wisconsin-Madison/Digestion - Cloning|Digestion - Cloning]]
-Ligation
+* [[Team:Wisconsin-Madison/Loading Gel Protocol|Loading Gel Protocol]]
-Digestion
+* [[Team:Wisconsin-Madison/Sequencing Reaction|Sequencing Reaction]]
-Inoculation of Plasmids in Liquid Media
+* [[Team:Wisconsin-Madison/Colony Polymerase Chain Reaction (PCR)|Colony Polymerase Chain Reaction (PCR)]]
-Inoculation of Cyanobacteria
+* [[Team:Wisconsin-Madison/Genomic DNA Extraction|Genomic DNA Extraction]]
-Loading Gel Protocol
+* [[Team:Wisconsin-Madison/Safety|Safety]]
-Plasmid Clean-up
-Gel Extraction from Agar
-Sequencing Reaction
-Colony Polymerase Chain Reaction (PCR)