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Team:UNICAMP-Brazil/Notebooks/August 21
From 2009.igem.org
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==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
| - | ==== | + | ====Recovered biobricks==== |
| - | + | Miniprep, digestion and ligation of RBS | |
| - | * After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol | + | * Today we perfomed miniprep from the RBS transformed ''E. coli''. The fragment size was correct and we decided to digest it ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase]) without purifying. It worked. |
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| + | * After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol 11. | ||
''Raíssa'' | ''Raíssa'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 02:16, 21 October 2009
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