Team:UNICAMP-Brazil/Notebooks/August 21

From 2009.igem.org

(Difference between revisions)
(→Recovered biobricks)
(→Recovered biobricks)
Line 8: Line 8:
====Recovered biobricks====
====Recovered biobricks====
-
Miniprep, digestion and ligation of RBS
+
'''Miniprep, digestion and ligation of RBS'''
* Today we perfomed miniprep from the RBS transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. The fragment size was correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14] without purifying. It worked.  
* Today we perfomed miniprep from the RBS transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. The fragment size was correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14] without purifying. It worked.  

Revision as of 02:22, 21 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

Recovered biobricks

Miniprep, digestion and ligation of RBS

  • Today we perfomed miniprep from the RBS transformed E. coli according to the Protocol 2. The fragment size was correct and we decided to digest it according to the Protocol 14 without purifying. It worked.
  • After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol 11.

Raíssa




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/August_21"