Team:UNICAMP-Brazil/Notebooks/August 21

(Difference between revisions)

Revision as of 06:18, 21 October 2009

Miniprep, digestion and ligation of RBS

Today we perfomed miniprep from the RBS transformed E. coli according to the Protocol 2. The fragment size seamed to be correct and we decided to digest it according to the Protocol 14 with the SpeI and PstI enzymes. It worked.

After the RBS plasmid digestion, we ligated it to CFP previously digested with XbaI and PstI according to the Protocol 11.

Raíssa

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 '''Miniprep, digestion and ligation of RBS'''
-* Today we perfomed miniprep from the RBS transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. The fragment size was correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]  with the ''Spe''I and ''Pst''I enzymes. It worked.
+* Today we perfomed miniprep from the RBS transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. The fragment size seamed to be correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]  with the ''Spe''I and ''Pst''I enzymes. It worked.
-* After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
+* After the RBS plasmid digestion, we ligated it to CFP previously digested with ''Xba''I and ''Pst''I according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
 [[Image:RBSdigestion.jpg‎|500px|center]]