Team:UNICAMP-Brazil/Notebooks/September 1

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YeastGuard

Gallus gallus cDNA synthesis

We decided to make cDNA synthesis of the 4 samples of RNA (Blood1, Blood3, Spleen1 and Oviduct2). We used both pDN6 and oligodT primers for the synthesis.

Taís

RBS+CFP ligation

We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed Protocol 14. The digestion lasted four hours and we used less plasmid comparing to the first digestion.

Wesley

@@ Line 13: / Line 13: @@
 ====RBS+CFP ligation====
-We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed Protocol Y (see protocols section). The digestion lasted four hours and we used less plasmid comparing to the first digestion.
+We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Restriction_reaction Protocol 14]. The digestion lasted four hours and we used less plasmid comparing to the first digestion.
 ''Wesley''
 {{:Team:UNICAMP-Brazil/inc_rodape}}