Team:TUDelft/Modeling Conjugation Plots

From 2009.igem.org

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{{Template:TUDelftiGEM2009_menu_Model}}
{{Template:TUDelftiGEM2009_menu_Model}}
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=Conjugation Modeling Plots=
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='''Conjugation Modeling Plots'''=
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Here is a selection of the various simulations made.
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==''SIM1''==
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==SIM1==
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Parameters used:  
Parameters used:  
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|}
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==SIM2==
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==''SIM2''==
Parameters used:  
Parameters used:  
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Ratio of Donors to Recipients = 1
Ratio of Donors to Recipients = 1
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 +
Animation of conjugation showing all colonies:
 +
[[Image:Gr30ng099_1hr_ani_all.gif|border]]
{|border = "0"
{|border = "0"
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|}
|}
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==SIM3==
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==''SIM3''==
Parameters used:  
Parameters used:  
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==SIM4==
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==''SIM4''==
Parameters used:  
Parameters used:  
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==SIM5==
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==''SIM5''==
Parameters used:  
Parameters used:  
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Ratio of Donors to Recipients = 1
Ratio of Donors to Recipients = 1
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 +
Animation of conjugation showing all colonies:
 +
[[Image:Gr60ng099_1hr_ani_all.gif|border]]
{|border = "0"
{|border = "0"
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==SIM6==
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==''SIM6''==
Parameters used:  
Parameters used:  
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|rowspan="4"|
|rowspan="4"|
<gallery widths="240px" perrow="2" heights="188px" >
<gallery widths="240px" perrow="2" heights="188px" >
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Image:Gr20ng099_1hrb1R.png | Recipients at T = 0 hr.
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Image:gr30ng099_1hr_ci1000b1R.png | Recipients at T = 0 hr.
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Image:Gr20ng099_1hrb1D.png | Donors at T = 0 hr.
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Image:gr30ng099_1hr_ci1000b1D.png | Donors at T = 0 hr.
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Image:Gr20ng099_1hrb1T.png | Transconjugants at T = 0.1 hr.
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Image:gr30ng099_1hr_ci1000b1T.png | Transconjugants at T = 0.1 hr.
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Image:Gr20ng099_1hrb2T.png | Transconjugants at T = 0.2 hr.
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Image:gr30ng099_1hr_ci1000b2T.png | Transconjugants at T = 0.2 hr.
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Image:Gr20ng099_1hrb3T.png | Transconjugants at T = 0.3 hr.
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Image:gr30ng099_1hr_ci1000b3T.png | Transconjugants at T = 0.3 hr.
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Image:Gr20ng099_1hrb4T.png | Transconjugants at T = 0.4 hr.
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Image:gr30ng099_1hr_ci1000b4T.png | Transconjugants at T = 0.4 hr.
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Image:Gr20ng099_1hrb5T.png | Transconjugants at T = 0.5 hr.
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Image:gr30ng099_1hr_ci1000b5T.png | Transconjugants at T = 0.5 hr.
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Image:Gr20ng099_1hrb6T.png | Transconjugants at T = 0.6 hr.
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Image:gr30ng099_1hr_ci1000b6T.png | Transconjugants at T = 0.6 hr.
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Image:Gr20ng099_1hrb7T.png | Transconjugants at T = 0.7 hr.
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Image:gr30ng099_1hr_ci1000b7T.png | Transconjugants at T = 0.7 hr.
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Image:Gr20ng099_1hrb8T.png | Transconjugants at T = 0.8 hr.
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Image:gr30ng099_1hr_ci1000b8T.png | Transconjugants at T = 0.8 hr.
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Image:Gr20ng099_1hrb9T.png | Transconjugants at T = 0.9 hr.
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Image:gr30ng099_1hr_ci1000b9T.png | Transconjugants at T = 0.9 hr.
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Image:Gr20ng099_1hrb10T.png | Transconjugants at T = 1.0 hr.
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Image:gr30ng099_1hr_ci1000b10T.png | Transconjugants at T = 1.0 hr.
</gallery>
</gallery>
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==SIM7==
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==''SIM7''==
Parameters used:  
Parameters used:  
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|rowspan="4"|
|rowspan="4"|
<gallery widths="240px" perrow="2" heights="188px" >
<gallery widths="240px" perrow="2" heights="188px" >
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Image:Gr20ng099_1hrb1R.png | Recipients at T = 0 hr.
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Image:gr30ng099_1hr_ci5000b1R.png | Recipients at T = 0 hr.
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Image:Gr20ng099_1hrb1D.png | Donors at T = 0 hr.
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Image:gr30ng099_1hr_ci5000b1D.png | Donors at T = 0 hr.
-
Image:Gr20ng099_1hrb1T.png | Transconjugants at T = 0.1 hr.
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Image:gr30ng099_1hr_ci5000b1T.png | Transconjugants at T = 0.1 hr.
-
Image:Gr20ng099_1hrb2T.png | Transconjugants at T = 0.2 hr.
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Image:gr30ng099_1hr_ci5000b2T.png | Transconjugants at T = 0.2 hr.
-
Image:Gr20ng099_1hrb3T.png | Transconjugants at T = 0.3 hr.
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Image:gr30ng099_1hr_ci5000b3T.png | Transconjugants at T = 0.3 hr.
-
Image:Gr20ng099_1hrb4T.png | Transconjugants at T = 0.4 hr.
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Image:gr30ng099_1hr_ci5000b4T.png | Transconjugants at T = 0.4 hr.
-
Image:Gr20ng099_1hrb5T.png | Transconjugants at T = 0.5 hr.
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Image:gr30ng099_1hr_ci5000b5T.png | Transconjugants at T = 0.5 hr.
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Image:Gr20ng099_1hrb6T.png | Transconjugants at T = 0.6 hr.
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Image:gr30ng099_1hr_ci5000b6T.png | Transconjugants at T = 0.6 hr.
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Image:Gr20ng099_1hrb7T.png | Transconjugants at T = 0.7 hr.
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Image:gr30ng099_1hr_ci5000b7T.png | Transconjugants at T = 0.7 hr.
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Image:Gr20ng099_1hrb8T.png | Transconjugants at T = 0.8 hr.
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Image:gr30ng099_1hr_ci5000b8T.png | Transconjugants at T = 0.8 hr.
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Image:Gr20ng099_1hrb9T.png | Transconjugants at T = 0.9 hr.
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Image:gr30ng099_1hr_ci5000b9T.png | Transconjugants at T = 0.9 hr.
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Image:Gr20ng099_1hrb10T.png | Transconjugants at T = 1.0 hr.
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Image:gr30ng099_1hr_ci5000b10T.png | Transconjugants at T = 1.0 hr.
</gallery>
</gallery>
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==SIM8==
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==''SIM8''==
Parameters used:  
Parameters used:  
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|rowspan="4"|
|rowspan="4"|
<gallery widths="240px" perrow="2" heights="188px" >
<gallery widths="240px" perrow="2" heights="188px" >
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Image:Gr20ng099_1hrb1R.png | Recipients at T = 0 hr.
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Image:gr30ng099_1hr_ci20000b1R.png | Recipients at T = 0 hr.
-
Image:Gr20ng099_1hrb1D.png | Donors at T = 0 hr.
+
Image:gr30ng099_1hr_ci20000b1D.png | Donors at T = 0 hr.
-
Image:Gr20ng099_1hrb1T.png | Transconjugants at T = 0.1 hr.
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Image:gr30ng099_1hr_ci20000b1T.png | Transconjugants at T = 0.1 hr.
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Image:Gr20ng099_1hrb2T.png | Transconjugants at T = 0.2 hr.
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Image:gr30ng099_1hr_ci20000b2T.png | Transconjugants at T = 0.2 hr.
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Image:Gr20ng099_1hrb3T.png | Transconjugants at T = 0.3 hr.
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Image:gr30ng099_1hr_ci20000b3T.png | Transconjugants at T = 0.3 hr.
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Image:Gr20ng099_1hrb4T.png | Transconjugants at T = 0.4 hr.
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Image:gr30ng099_1hr_ci20000b4T.png | Transconjugants at T = 0.4 hr.
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Image:Gr20ng099_1hrb5T.png | Transconjugants at T = 0.5 hr.
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Image:gr30ng099_1hr_ci20000b5T.png | Transconjugants at T = 0.5 hr.
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Image:Gr20ng099_1hrb6T.png | Transconjugants at T = 0.6 hr.
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Image:gr30ng099_1hr_ci20000b6T.png | Transconjugants at T = 0.6 hr.
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Image:Gr20ng099_1hrb7T.png | Transconjugants at T = 0.7 hr.
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Image:gr30ng099_1hr_ci20000b7T.png | Transconjugants at T = 0.7 hr.
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Image:Gr20ng099_1hrb8T.png | Transconjugants at T = 0.8 hr.
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Image:gr30ng099_1hr_ci20000b8T.png | Transconjugants at T = 0.8 hr.
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Image:Gr20ng099_1hrb9T.png | Transconjugants at T = 0.9 hr.
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Image:gr30ng099_1hr_ci20000b9T.png | Transconjugants at T = 0.9 hr.
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Image:Gr20ng099_1hrb10T.png | Transconjugants at T = 1.0 hr.
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Image:gr30ng099_1hr_ci20000b10T.png | Transconjugants at T = 1.0 hr.
</gallery>
</gallery>
|}
|}
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==SIM9==
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==''SIM9''==
Parameters used:  
Parameters used:  
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|rowspan="4"|
|rowspan="4"|
<gallery widths="240px" perrow="2" heights="188px" >
<gallery widths="240px" perrow="2" heights="188px" >
-
Image:Gr20ng099_1hrb1R.png | Recipients at T = 0 hr.
+
Image:gr30ng099_1hr_ci40000b1R.png | Recipients at T = 0 hr.
-
Image:Gr20ng099_1hrb1D.png | Donors at T = 0 hr.
+
Image:gr30ng099_1hr_ci40000b1D.png | Donors at T = 0 hr.
-
Image:Gr20ng099_1hrb1T.png | Transconjugants at T = 0.1 hr.
+
Image:gr30ng099_1hr_ci40000b1T.png | Transconjugants at T = 0.1 hr.
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Image:Gr20ng099_1hrb2T.png | Transconjugants at T = 0.2 hr.
+
Image:gr30ng099_1hr_ci40000b2T.png | Transconjugants at T = 0.2 hr.
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Image:Gr20ng099_1hrb3T.png | Transconjugants at T = 0.3 hr.
+
Image:gr30ng099_1hr_ci40000b3T.png | Transconjugants at T = 0.3 hr.
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Image:Gr20ng099_1hrb4T.png | Transconjugants at T = 0.4 hr.
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Image:gr30ng099_1hr_ci40000b4T.png | Transconjugants at T = 0.4 hr.
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Image:Gr20ng099_1hrb5T.png | Transconjugants at T = 0.5 hr.
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Image:gr30ng099_1hr_ci40000b5T.png | Transconjugants at T = 0.5 hr.
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Image:Gr20ng099_1hrb6T.png | Transconjugants at T = 0.6 hr.
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Image:gr30ng099_1hr_ci40000b6T.png | Transconjugants at T = 0.6 hr.
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Image:Gr20ng099_1hrb7T.png | Transconjugants at T = 0.7 hr.
+
Image:gr30ng099_1hr_ci40000b7T.png | Transconjugants at T = 0.7 hr.
-
Image:Gr20ng099_1hrb8T.png | Transconjugants at T = 0.8 hr.
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Image:gr30ng099_1hr_ci40000b8T.png | Transconjugants at T = 0.8 hr.
-
Image:Gr20ng099_1hrb9T.png | Transconjugants at T = 0.9 hr.
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Image:gr30ng099_1hr_ci40000b9T.png | Transconjugants at T = 0.9 hr.
-
Image:Gr20ng099_1hrb10T.png | Transconjugants at T = 1.0 hr.
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Image:gr30ng099_1hr_ci40000b10T.png | Transconjugants at T = 1.0 hr.
</gallery>
</gallery>

Latest revision as of 20:15, 21 October 2009

Conjugation Modeling Plots

Here is a selection of the various simulations made.

SIM1

Parameters used:

gr = 20 μm/hr

λ = 5.76 CFU/mm2 (Nd = 10000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.

SIM2

Parameters used:

gr = 30 μm/hr

λ = 5.76 CFU/mm2 (Nd = 10000)

Ratio of Donors to Recipients = 1

Animation of conjugation showing all colonies: Gr30ng099 1hr ani all.gif

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.

SIM3

Parameters used:

gr = 40 μm/hr

λ = 5.76 CFU/mm2 (Nd = 10000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.


SIM4

Parameters used:

gr = 50 μm/hr

λ = 5.76 CFU/mm2 (Nd = 10000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.


SIM5

Parameters used:

gr = 60 μm/hr

λ = 5.76 CFU/mm2 (Nd = 10000)

Ratio of Donors to Recipients = 1

Animation of conjugation showing all colonies: Gr60ng099 1hr ani all.gif

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.


SIM6

Parameters used:

gr = 30 μm/hr

λ = 1.44 CFU/mm2 (Nd = 1000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.


SIM7

Parameters used:

gr = 30 μm/hr

λ = 2.88 CFU/mm2 (Nd = 5000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.


SIM8

Parameters used:

gr = 20 μm/hr

λ = 11.53 CFU/mm2 (Nd = 20000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.

SIM9

Parameters used: gr = 20 μm/hr

λ = 23.06 CFU/mm2 (Nd = 40000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.

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