Team:TUDelft/Modeling Conjugation Plots

From 2009.igem.org

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=Conjugation Modeling Plots=
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='''Conjugation Modeling Plots'''=
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Here is a selection of the various simulations made.
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==''SIM1''==
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==SIM1==
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==SIM2==
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==SIM3==
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==SIM4==
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==SIM5==
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==SIM6==
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==SIM7==
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==SIM8==
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==SIM9==
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Latest revision as of 20:15, 21 October 2009

Conjugation Modeling Plots

Here is a selection of the various simulations made.

SIM1

Parameters used:

gr = 20 μm/hr

λ = 5.76 CFU/mm2 (Nd = 10000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.

SIM2

Parameters used:

gr = 30 μm/hr

λ = 5.76 CFU/mm2 (Nd = 10000)

Ratio of Donors to Recipients = 1

Animation of conjugation showing all colonies: Gr30ng099 1hr ani all.gif

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.

SIM3

Parameters used:

gr = 40 μm/hr

λ = 5.76 CFU/mm2 (Nd = 10000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.


SIM4

Parameters used:

gr = 50 μm/hr

λ = 5.76 CFU/mm2 (Nd = 10000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.


SIM5

Parameters used:

gr = 60 μm/hr

λ = 5.76 CFU/mm2 (Nd = 10000)

Ratio of Donors to Recipients = 1

Animation of conjugation showing all colonies: Gr60ng099 1hr ani all.gif

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.


SIM6

Parameters used:

gr = 30 μm/hr

λ = 1.44 CFU/mm2 (Nd = 1000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.


SIM7

Parameters used:

gr = 30 μm/hr

λ = 2.88 CFU/mm2 (Nd = 5000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.


SIM8

Parameters used:

gr = 20 μm/hr

λ = 11.53 CFU/mm2 (Nd = 20000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.

SIM9

Parameters used: gr = 20 μm/hr

λ = 23.06 CFU/mm2 (Nd = 40000)

Ratio of Donors to Recipients = 1

Recipients at T = 0 hr.

Donors at T = 0 hr.

Transconjugants at T = 0.1 hr.

Transconjugants at T = 0.2 hr.

Transconjugants at T = 0.3 hr.

Transconjugants at T = 0.4 hr.

Transconjugants at T = 0.5 hr.

Transconjugants at T = 0.6 hr.

Transconjugants at T = 0.7 hr.

Transconjugants at T = 0.8 hr.

Transconjugants at T = 0.9 hr.

Transconjugants at T = 1.0 hr.

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